iPRM is a multiplexed LC/MS assay service that combines the specificity of mass spectrometry, the throughput of an immunoassay, and the power of multiplexing to accelerate the time-to-market of your drug and/or diagnostic test. Our iPRM services let you leverage the proteomic expertise of CST scientists and the established specificity and sensitivity of CST antibodies for streamlined assay development and reliable validation of your therapeutic target(s). iPRM services let you:
Learn more about iPRM services or consult with a CST in-house expert.
Lysates from a wide variety of research, clinical, or preclinical samples, including core needle biopsies, cells, or tissues, are proteolytically digested and assay targets are antibody-enriched along with quantified heavy-isotype peptide standards chemically identical to the targets of the assay. The enriched sample is analyzed on a Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (MS) using a targeted PRM scan to precisely quantify the target and standard peptides with the highest sensitivity. Data are provided in Microsoft® Excel® and PowerPoint®.
Pick from our list of ready-to-go assays below. These assays are pre validated and use CST components, including the high-affinity antibody and in-house synthesized AQUA peptide standards.
|Catalog #||Protein Site||Peptide||LLOD (fmol/100 μg)||LLOQ (fmol/100 μg)|
LLOD and LLOQ were determined based on limit of blank, variance of the blank sample and variance of the lowest level spike-in sample.
If we don’t have the assay you’re looking for yet, we’re happy to develop it. A CST proteomics expert will work with you to define a custom assay. CST then develops and validates the assay in-house. If you’re interested in a target we don’t currently offer, we can collaborate with you to develop a custom antibody with the specificity and sensitivity CST antibodies are known for. Externally developed antibodies can also be used.
|Average intra-assay (within day) CV||Average inter-assay (between day) CV||Total CV √(intraCV²+interCV²)||n=|