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4801
Phospho-SMC1 (Ser957) Antibody
Primary Antibodies

Phospho-SMC1 (Ser957) Antibody #4801

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# Product Name Application Reactivity
  • WB
  • IF
H M

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 145
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-SMC1 (Ser957) Antibody detects endogenous levels of SMC1 only when phosphorylated at Ser957.

Species predicted to react based on 100% sequence homology:

Xenopus, Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser957 of human SMC1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).

  1. Michaelis, C. et al. (1997) Cell 91, 35-45.
  2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
  3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
  4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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