Revision 6

#2794

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P, IF-F, IF-IC

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
37

Source/Isotype:
Rabbit IgG

UniProt ID:
#O00767

Entrez-Gene Id:
6319

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:100 - 1:400
Immunofluorescence (Frozen) 1:100
Immunofluorescence (Immunocytochemistry) 1:100 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #38672.

Specificity/Sensitivity

SCD1 (C12H5) Rabbit Monoclonal Antibody detects endogenous levels of total SCD1 protein. Species cross-reactivity for IHC-P is mouse only.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu35 of mouse SCD1.

Background

Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl–CoA and stearoyl–CoA to palmitoleoyl–CoA (16:1) and oleoyl–CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids, and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to the plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technologyロゴ
Western blot analysis of 3T3-L1 cell lysates (differentiated into adipocytes) using SCD1 (C12H5) Rabbit mAb.
Western Blotting Image 1: SCD1 (C12H5) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells undifferentiated (left) or differentiated (right) using SCD1 (C12H5) Rabbit mAb.
Immunohistochemistry Image 1: SCD1 (C12H5) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse lung using SCD1 (C12H5) Rabbit mAb (left) or without primary antibody (right).
Immunohistochemistry Image 2: SCD1 (C12H5) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded mouse brown fat using SCD1 (C12H5) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemistry Image 3: SCD1 (C12H5) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse brown fat using SCD1 (C12H5) Rabbit mAb.
Immunohistochemistry Image 4: SCD1 (C12H5) Rabbit Monoclonal Antibody
Immunohistochemical analysis of parafin-embedded mouse thymus with staining of adjacent adipose tissue using SCD1 (C12H5) Rabbit mAb.
Immunohistochemistry Image 5: SCD1 (C12H5) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technologyロゴ
Immunohistochemical analysis of parafin-embedded mouse prostate with staining of adjacent adipose tissue using SCD1 (C12H5) Rabbit mAb.
Immunohistochemistry Image 6: SCD1 (C12H5) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse lung labeled with SCD1 (C12H5) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate) #81143 (right, magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 1: SCD1 (C12H5) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with SCD1 (C12H5) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate) #81143 (right, magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 2: SCD1 (C12H5) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technologyロゴ
Confocal immunofluorescent analysis of fixed frozen mouse brown adipose tissue labeled with SCD1 (C12H5) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate) #81143 (right, magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 3: SCD1 (C12H5) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of NIH/3T3-L1 cells, using SCD1 (C12H5) Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunofluorescence Image 1: SCD1 (C12H5) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.