Revision 1

#40354

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
80-100

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9NQS3

Entrez-Gene Id:
25945

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody recognizes endogenous levels of total nectin-3/PVRL3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val521 of human nectin-3/PVRL3 protein.

Background

Nectin cell adhesion molecule 3 (Nectin-3), also known as poliovirus receptor-related protein 3 (PVRL3) and CD113, is a member of the immunoglobulin-like cellular adhesion molecule family and plays an important role in calcium-independent cellular adhesion (1,2). There are four nectin family members, nectin-1, nectin-2, nectin-3, and nectin-4. They are composed of an ectodomain containing three tandem Ig-like regions arranged as an N-terminal variable-like domain followed by two constant-like domains, a single transmembrane region, and a cytoplasmic domain that binds to the actin cytoskeleton through the adaptor protein afadin (3,4). Nectin-3 can engage in homophilic, as well as heterophilic cell adhesion by engaging with other nectin family members (5). In the nervous system, nectin-3 expression is associated with chronic stress-induced synaptic abnormalities and has been proposed as a target for stress-related cognitive disorder treatment (6,7). Nectin-3 can also interact with the immune system. Nectin-3 expressed on antigen-presenting cells (APCs) and tumor cells can act as a ligand for the inhibitory receptor TIGIT, expressed on activated T cell subsets and natural killer (NK) cells (8). Aberrant expression of nectin-3 has been observed in multiple cancers, including breast, pancreatic, lung, colorectal, and ovarian cancer (9-14).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from OVCAR-3, LN-18, and SW620 cells using Nectin-3/PVRL3 (F9R9R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of nectin-3/PVRL3 protein in SW620 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human nectin-3/PVRL3 (hNectin-3/PVRL3-Myc/DDK; +), using Nectin-3/PVRL3 (F9R9R) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody
Immunoprecipitation of nectin-3/PVRL3 protein from LN-18 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Nectin-3/PVRL3 (F9R9R) Rabbit mAb. Western blot analysis was performed using Nectin-3/PVRL3 (F9R9R) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: Nectin-3/PVRL3 (F9R9R) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.