Revision 4

#58145

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IHC-P, IF-F, IF-IC, FC-FP

Reactivity:
M R

Sensitivity:
Endogenous

MW (kDa):
100

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q61391

Entrez-Gene Id:
17380

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunohistochemistry (Paraffin) 1:50
Immunofluorescence (Frozen) 1:200 - 1:800
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #67330.

Specificity/Sensitivity

CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD10/Neprilysin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10/Neprilysin protein.

Background

CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10+GPR77+ carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technologyロゴ
Western blot analysis of extracts from various cell lines and tissues using CD10/Neprilysin (F4P6H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of CD10/Neprilysin protein in NIH/3T3 and MBT2 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Simple WesternTM analysis of lysates (0.1mg/mL) from mIMCD-3 cells using CD10/Neprilysin (F4P6H) Rabbit mAb #58145. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.  The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody.  This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Western Blotting Image 1: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD10/Neprilysin (F4P6H) Rabbit mAb.
Immunohistochemistry Image 1: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technologyロゴ

Immunohistochemical analysis of paraffin-embedded MC-38 syngeneic tumor using CD10/Neprilysin (F4P6H) Rabbit mAb.

Immunohistochemistry Image 2: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse lung using CD10/Neprilysin (F4P6H) Rabbit mAb.

Immunohistochemistry Image 3: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse testis using CD10/Neprilysin (F4P6H) Rabbit mAb.
Immunohistochemistry Image 4: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD10/Neprilysin (F4P6H) Rabbit mAb.
Immunohistochemistry Image 5: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse oviduct using CD10/Neprilysin (F4P6H) Rabbit mAb.

Immunohistochemistry Image 6: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse thymus using CD10/Neprilysin (F4P6H) Rabbit mAb.
Immunohistochemistry Image 7: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD10/Neprilysin (F4P6H) Rabbit mAb (left) or a CD10/Neprilysin Rabbit mAb (right). These two antibodies detect unique, non-overlapping epitopes on CD10/Neprilysin. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 8: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse liver using CD10/Neprilysin (F4P6H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 9: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded Renca cell pellet (left, positive) or MBT2 cell pellet (right, negative) using CD10/Neprilysin (F4P6H) Rabbit mAb.
Immunohistochemistry Image 10: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technologyロゴ
Confocal immunofluorescent analysis of fixed frozen mouse kidney labeled with CD10/Neprilysin (F4P6H) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 555 Conjugate) #19581 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 1: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with CD10/Neprilysin (F4P6H) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 555 Conjugate) #19581 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 2: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of mIMCD-3 cells (left, positive) and NIH/3T3 cells (right, negative) using CD10/Neprilysin (F4P6H) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technologyロゴ
Flow cytometric analysis of fixed/permeabilized NIH/3T3 cells (blue, negative) and Renca cells (green, positive) using CD10/Neprilysin (F4P6H) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: CD10/Neprilysin (F4P6H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.