Revision 3

#72565

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
18

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9NR55

Entrez-Gene Id:
55509

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #68711.

Specificity/Sensitivity

BATF3 (E3F3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total BATF3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BATF3 protein.

Background

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3, p21SNFT) is a member of the basic leucine zipper family (1,2,5) which dimerizes with Jun proteins and represses activator protein-1 (AP-1) activity (1). BATF3 is required for development of conventional type 1 dendritic cells (cDC1s), which cross-present antigens in the context of MHC class I to naïve CD8+ T cells, priming them to become activated cytotoxic CD8+ T cells (2-4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Western blot analysis of extracts from various cell lines using BATF3 (E3F3L) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of BATF3 protein in T84 and Hep G2 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human BATF3 protein (hBATF3-Myc/DDK; +), using BATF3 (E3F3L) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Immunoprecipitation of BATF3 protein from KARPAS 299 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BATF3 (E3F3L) Rabbit mAb. Western blot analysis was performed using BATF3 (E3F3L) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded human Hodgkin lymphoma using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 1: BATF3 (E3F3L) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using BATF3 (E3F3L) Rabbit mAb.

Immunohistochemistry Image 2: BATF3 (E3F3L) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BATF3 (E3F3L) Rabbit mAb.

Immunohistochemistry Image 3: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 4: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 5: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human tonsil using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 6: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded normal human thymus using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 7: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human lymph node using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 8: BATF3 (E3F3L) Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded normal human esophagus using BATF3 (E3F3L) Rabbit mAb.

Immunohistochemistry Image 9: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ

Immunohistochemical analysis of paraffin-embedded human appendix using BATF3 (E3F3L) Rabbit mAb.

Immunohistochemistry Image 10: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human small intestine using BATF3 (E3F3L) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 11: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded KARPAS 299 cell pellet (left, positive) or T84 cell pellet (right, negative) using BATF3 (E3F3L) Rabbit mAb.
Immunohistochemistry Image 12: BATF3 (E3F3L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.