Revision 3

#89348

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IHC-P

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
51-60

Source/Isotype:
Rabbit IgG

UniProt ID:
#P19544

Entrez-Gene Id:
7490

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:200
Immunohistochemistry (Paraffin) 1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

WT1 (F3T7B) Rabbit mAb (Carboxy-terminal Antigen) recognizes endogenous levels of total WT1 protein.

This antibody is recommended for the detection of fusion proteins containing the C-terminus region of WT1 found in desmoplastic small round cell tumors by immunohistochemistry. For the detection of wild-type WT1, it is recommended to use WT1 (D8I7F) XP® Rabbit mAb #83535.

Non-specific staining was observed in kidney, liver, and small intestine by immunohistochemistry.

Species predicted to react based on 100% sequence homology

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human WT1 protein.

Background

Wilms' Tumor 1 (WT1) is a transcription factor named from Wilms' Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the WT1 gene (1). It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of WT1 expression result in a variety of syndromes affecting the genitourinary system and other tissues (2-5).

WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post-translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post-translational modifications of the protein (9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

使用に関する制限

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human WT1 (Myc/DDK +) using WT1 (F3T7B) Rabbit mAb (Carboxy-terminal Antigen) (upper), Myc-Tag (71D10) Rabbit mAb 2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 1: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Western blot analysis of extracts from various cell lines using WT1 (F3T7B) Rabbit mAb (Carboxy-terminal Antigen) (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of WT1 protein in HeLa cells is consistent with the predicted expression pattern.
Western Blotting Image 2: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Simple WesternTM analysis of lysates (1.0 mg/mL) from COV434 cells using WT1 (F3T7B) Rabbit mAb (Carboxy-terminal Antigen) #89348.  The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody.  The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody.  This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Western Blotting Image 1: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded human desmoplastic small round cell tumor of the abdomen
(metastatic site) using WT1 (F3T7B) Rabbit mAb.
Immunohistochemistry Image 1: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Immunohistochemical analysis of paraffin-embedded human soft tissue desmoplastic small round cell tumor using WT1 (F3T7B) Rabbit mAb #89348 (left) and WT1 (D8I7F) XP® Rabbit mAb #83535 (right). Note the lack of tumor staining observed with WT1 (D8I7F) XP® Rabbit mAb #83535 as this antibody does not detect the fusion protein located at the C-terminus of WT1.
Immunohistochemistry Image 2: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Immunohistochemical analysis of paraffin-embedded human desmoplastic small round cell tumor of the abdomen (metastatic site) using WT1 (F3T7B) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Immunohistochemistry Image 3: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technologyロゴ
Immunohistochemical analysis of paraffin-embedded OVCAR-3 (NIH) cell pellet (left, positive) or DU145 cell pellet (right, negative) using WT1 (F3T7B) Rabbit mAb.
Immunohistochemistry Image 4: WT1 (F3T7B) Rabbit Monoclonal Antibody (Carboxy-terminal Antigen)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.