Western blot analysis of extracts from HeLa cells, untreated (-) or treated with TPA #4174 (200 nM, 20 min; +) with or without pretreating the cells with PD 0325901 for 1 hour at the indicated concentrations, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper), p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Chemical structure of PD 0325901.
PD 0325901 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.03 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 482.2 g/mol
Molecular Formula: C16H14F3IN2O4
Solubility: Soluble in DMSO at 12 mg/ml.
PD 0325901 is a potent and selective non-ATP competitive MEK inhibitor. This small molecule is highly effective at inhibiting cell growth and proliferation in various cancer cells (1). The potency of PD 0325901 has been illustrated by the inhibition of ERK phosphorylation in mouse and human cell models with an IC50 of 0.33 nM and between 20-50 nM, respectively (1,2). PD 0325901 increases the self-renewal of mouse embryonic stem cells (mESCs) by enhancing the expression of pluripotency genes and acts as an antagonist for retinoic acid (RA) induced cell differentiation (3).
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