PathScan® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout) (#13047) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

UniProt ID:

#P40763, #P01579, #P29459, #P22301, #P35225, #P05112, #P05231, #Q16552, #P60568, #P04141, #P05113, #P10145, #P01375

Entrez-Gene Id:

6774, 3458, 3592, 3586, 3596, 3565, 3569, 3605, 3558, 1437, 3567, 3576, 7124

Product Information

Storage

Kit should be stored at 4°C with the exception of Lysis Buffer, which is stored at –20°C (packaged separately).

Specificity / Sensitivity

PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout) detects the target proteins as specified on the Array Target Map (Figure 1). No significant cross reactivity has been observed between targets. This kit is optimized for cell culture supernatants. Recommended starting cell culture supernatant range is 20-75 μl. All antibodies have been validated for human-derived cell culture supernatants.

Species Reactivity:

Human

Product Description

The PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Chemiluminescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. This array kit allows for the simultaneous detection of 12 unique extracellular signaling molecules. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing the user to test up to 32 different samples and generate 384 data points in a single experiment. Cell supernatant is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated HRP and LumiGLO^® Reagent are then used to visualize the bound detection antibody by chemiluminescence. An image of the slide can be captured with either a digital imaging system or standard chemiluminescent film. The image can be analyzed visually or the spot intensities quantified using array analysis software.

Background

Cytokines are secreted intercellular signaling molecules that regulate many biological processes including inflammation, host defense, and cell differentiation. Cytokine profiles may provide insight into the molecular mechanisms that distinguish between healthy and diseased states. The PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit offers an antibody panel against a broad array of cytokines to enable measurement of their relative changes in cell culture supernatants.

Upon activation, naive CD4+ helper T cells differentiate into distinct functional subsets. The development of these subsets is driven, in part, by the cytokine milieu. Type 1 (Th1) cells help drive cellular immunity against intracellular pathogens. IL-12 and IFN-γ induce Th1 cell development. Th1 cells produce IFN-γ and IL-2, which provide a positive feedback loop to enhance Th1 cell differentiation and NK cell and CD8+ T cell cytolytic activity.

Th2 cells play a crucial role in the humoral immune response against extracellular pathogens. IL-4 drives development of Th2 cells, which subsequently produce IL-4, IL-5, and IL-13. These cytokines induce B cell proliferation, antibody production, IgE class switching, and activate eosinophils respectively.

Another distinct helper T cell lineage, Th17, is important for mucosal immunity. Dysregulation of Th17 may significantly contribute to the development of autoimmunity. IL-17 produced by Th17 cells induces secretion of pro-inflammatory cytokines IL-6, IL-8, GM-CSF, and TNF-α. Many of these molecules link innate and adaptive immunity through the recruitment and activation of innate immune cells.

Effective immune responses require finely tuned coordination between pro and anti-inflammatory signals. Pro-inflammatory molecules play important roles in activating key immune players to fight infection. IL-8 induces granulocyte migration and activates neutrophil phagocytic activity. GM-CSF mobilizes monocytes into infected tissue and activates macrophage and neutrophils. TNF-α is a multifunctional pro-inflammatory cytokine involved with a number of processes including cell proliferation, differentiation, and apoptosis.

Uncontrolled inflammation may damage surrounding host tissue. IL-10 is a prototypical anti-inflammatory cytokine that serves to terminate the acute inflammatory response by inhibiting Th1 cell function and pro-inflammatory cytokine production.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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