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45289
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 700 Conjugate)
Antibody Conjugates
Monoclonal Antibody

Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 700 Conjugate) #45289

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Flow cytometric analysis of live human peripheral blood mononuclear cells using CD45RO (UCHL1) Mouse mAb (Alexa Fluor® 700 Conjugate) #94233 (solid line) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 700 Conjugate) (dashed line).

To Purchase # 45289S
製品番号 サイズ 価格 在庫
45289S
100 µl  (50 tests)

Supporting Data

Concentration 100 ug/ml
SENSITIVITY Endogenous
Source/Isotype Mouse IgG1, kappa

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 700 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells.

Product Usage Information

Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.

Directions for Use: Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of mouse IgG1 isotype control are used.

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 700 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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