Flow cytometric analysis of live human peripheral blood mononuclear cells using CD45RO (UCHL1) Mouse mAb (Alexa Fluor® 700 Conjugate) #94233 (solid line) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 700 Conjugate) (dashed line).
|Source/Isotype||Mouse IgG1, kappa|
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 700 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions for Use.Directions for Use: Important! Dilute this control antibody to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of mouse IgG1 isotype control are used.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 700 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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