|200 ug/ml||Endogenous||Mouse IgG1|
Flow cytometric analysis of live human peripheral blood mononuclear cells using CD14 (61D3) Mouse mAb (redFluor™ 710 Conjugate) #64342 (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control(redFluor™ 710 Conjugate) (dashed line). Analysis was performed on cells in the monocyte gate.Learn more about how we get our images.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.
Important for Use: Important: Dilute this control antibody to the same concentration (not dilution) as specific antibody in analysis. Higher background fluorescence may result if excessive amounts of mouse IgG isotype control is used. Do not use this antibody at 1:50 or 1:20 dilution like other CST redFluor™ 710 conjugated antibodies.
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
The antibody is not directed against any known antigen. It functions as an isotype control for mouse monoclonal antibodies.
This Cell Signaling Technology antibody is conjugated to redFluor™ 710 under optimal conditions and tested in-house for direct flow cytometric analysis in human cells.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. redFluor is a registered trademark of Tonbo Biosciences.
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