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4759
ER Protein Folding Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

ER Protein Folding Antibody Sampler Kit #4759

Citations (3)
Flow cytometric analysis of Hep G2 cells using ERp72 (D70D12) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from various cell types using Grp94 Antibody.
Western blot analysis of extracts from various cell types using ERp57 (G117) Antibody.
Western blot analysis of extracts from various cell lines using BiP (C50B12) Rabbit mAb.
Western blot analysis of extracts from various cell lines, using Ero1-Lα Antibody.
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.
Western blot analysis of extracts from various cell types using ERp44 (D17A6) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines using ERp72 (D70D12) XP® Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Immunohistochemical analysis of paraffin-embedded human glioblastoma using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERp44 (D17A6) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Confocal immunofluorescent analysis of PANC-1 cells using ERp72 (D70D12) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using BiP (C50B12) Rabbit mAb in the presence of control peptide (left) or BiP Blocking Peptide #1084 (right).
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of A204 cells using BiP (C50B12) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 4759
Cat. # Size Qty. Price Inventory
4759T
1 Kit  (7 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
BiP (C50B12) Rabbit mAb 3177 20 µl
  • WB
  • IHC
  • F
H M 78 Rabbit IgG
Ero1-Lα Antibody 3264 20 µl
  • WB
H 60 Rabbit 
ERp44 (D17A6) XP® Rabbit mAb 3798 20 µl
  • WB
  • IHC
H M R Mk 44 Rabbit IgG
ERp57 (G117) Antibody 2881 20 µl
  • WB
H M R 57 Rabbit 
ERp72 (D70D12) XP® Rabbit mAb 5033 20 µl
  • WB
  • IF
  • F
H M R Mk 72 Rabbit IgG
Grp94 Antibody 2104 20 µl
  • WB
H M R Mk 100 Rabbit 
PDI (C81H6) Rabbit mAb 3501 20 µl
  • WB
  • IHC
  • IF
H M R Mk 57 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The ER Protein Folding Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

Each antibody in the ER Protein Folding Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Gly584 of human BiP, the sequence of human ERp44, the residues surrounding Met279 of human ERp72 protein and the sequence of human PD1. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequences around Gly117 of human ERp57, Met622 of human Grp94 and Leu218 of human Ero1-Lα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

After their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress proteins 72, 57 and 44 (ERp72, ERp57 and ERp44), constitute the PDI family (4,5,6). The ER also contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (7) with sequence homology to Hsp90 (8). BiP is another chaperone whose synthesis is increased when protein folding is disturbed. BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (9).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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