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9869
Cyclin Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cyclin Antibody Sampler Kit #9869

Citations (32)
Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb #3741.
To Purchase # 9869
Cat. # Size Qty. Price Inventory
9869T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cyclin A2 (BF683) Mouse mAb 4656 20 µl
  • WB
H 55 Mouse IgE
Cyclin B1 Antibody 4138 20 µl
  • WB
  • IF
H M R Hm Mk 55 Rabbit 
Cyclin D1 (92G2) Rabbit mAb 2978 20 µl
  • WB
  • IHC
H M R 36 Rabbit IgG
Cyclin D2 (D52F9) Rabbit mAb 3741 20 µl
  • WB
  • IP
H M R 31 Rabbit IgG
Cyclin D3 (DCS22) Mouse mAb 2936 20 µl
  • WB
  • IHC
H M R 31 Mouse IgG1
Cyclin E1 (HE12) Mouse mAb 4129 20 µl
  • WB
H Mk 48 Mouse IgG1
Cyclin E2 Antibody 4132 20 µl
  • WB
H 48 Rabbit 
Cyclin H Antibody 2927 20 µl
  • WB
  • IP
H M R 36 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.

Specificity / Sensitivity

Each antibody in the Cyclin Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other cyclin proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin D1, the amino terminus of cyclin D2, recombinant human cyclin E1, or cyclin A2. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin H, cyclin E2, or the amino terminus of cyclin B. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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