Revision 6

#41608Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
β-Amyloid (D54D2) XP® Rabbit mAb 8243 20 µl 5 kDa Rabbit IgG
β-Amyloid (D3D2N) Mouse mAb 15126 20 µl 5 kDa Mouse IgG1
NeuN (D4G4O) XP® Rabbit mAb 24307 20 µl 46-55 kDa Rabbit IgG
Synaptophysin (7H12) Mouse mAb (IF Formulated) 9020 20 µl Mouse IgG1
PSD95 (D27E11) XP® Rabbit mAb 3450 20 µl 95 kDa Rabbit IgG
Cleaved Caspase-3 (Asp175) Antibody 9661 20 µl 17, 19 kDa Rabbit 
Cleaved PARP (Asp214) (D6X6X) Rabbit mAb 94885 20 µl 89 kDa Rabbit IgG
GFAP (E6N9L) Mouse mAb 34001 20 µl 50 kDa Mouse IgG2a
HS1 (D5A9) XP® Rabbit mAb 3892 20 µl 80 kDa Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit provides an economical means of detecting proteins to confirm neuronal viability and surrounding astrocytes and microglia in mouse models by immunofluorescence.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques, a major pathological hallmark of Alzheimer’s disease (1). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (2). Glial fibrillary acidic protein (GFAP) is the main intermediate filament in mature brain astroglial and radial glial cells and GFAP also plays an important role in modulating astroglial motility and shape (3). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (4). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 to be a useful and specific tool to study microglia (5). Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that occurs in presynaptic vesicles of neurons (6). Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (7,8). Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, including nuclear enzyme poly (ADP-ribose) polymerase (PARP) (9). PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (10). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (11).

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Mullen, R.J. et al. (1992) Development 116, 201-11.
  3. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  4. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  5. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  6. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci U S A 83, 3500-4.
  7. Cao, J. et al. (2005) J Cell Biol 168, 117-26.
  8. Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.
  9. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  10. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.
  11. Oliver, F.J. et al. (1998) J Biol Chem 273, 33533-9.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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    研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Revision 6
    #41608

    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit

    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 1 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from mouse brain using PSD95 (D27E11) XP® Rabbit mAb #3450. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 2 Expand Image
    Immunoprecipitation of cleaved caspase-3 from Jurkat extracts treated with Etoposide #2200 (25 mM; 5 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cleaved Caspase-3 (Asp175) Antibody. Western blot analysis was performed using Cleaved Caspase-3 (Asp175) Antibody. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 3 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) Antibody #9661. The virtual lane view (left) shows two target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 4 Expand Image
    Western blot analysis of human Aβ-37, Aβ-40, and Aβ-42 peptides using β-Amyloid (D3D2N) Mouse mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 5 Expand Image
    Western blot analysis of extracts from mouse brain, C2C12 cells, and rat brain using NeuN (D4G4O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 6 Expand Image
    Western blot analysis of extracts from various tissues and cell lines using GFAP (E6N9L) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 7 Expand Image
    Western blot analysis of extracts from human cerebellum and rat brain using PSD95 (D27E11) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 8 Expand Image
    Western blot analysis of cell extracts from Baf3, 32D, and mouse spleen using HS1 (D5A9) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 9 Expand Image
    Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 10 Expand Image
    Confocal immunofluorescent analysis of mouse brain (left) and mouse retina (right) using Synaptophysin (7H12) Mouse mAb (IF Formulated) (green) and CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #5715 (red, left). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 11 Expand Image
    Western blot analysis of extracts from serum-starved Neuro-2a cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 12 Expand Image
    Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (3hrs, 1 µM in vivo) or cytochrome c-treated (1hr, 0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 13 Expand Image
    Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (D3D2N) Mouse mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 14 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum using NeuN (D4G4O) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 15 Expand Image
    Immunohistochemical analysis of paraffin-embedded cynomolgus monkey brain using NeuN (D4G4O) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 16 Expand Image
    Western blot analysis of extracts from mouse brain using GFAP (E6N9L) Mouse mAb as the primary antibody. Various anti-mouse isotype specific antibodies (upper) and Anti-mouse IgG, HRP-linked Antibody #7076 (lower) were used as secondary antibodies.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 17 Expand Image
    Confocal immunofluorescent analysis of rat cerebellum and retina using PSD95 (D27E11) XP® Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 18 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse spleen using HS1 (D5A9) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 19 Expand Image
    Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 20 Expand Image
    Western blot analysis of extracts from serum-starved H-4-II-E cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 6 hr; +), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 21 Expand Image
    Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic and perinuclear localization in apoptotic cells (low and high magnification), using Cleaved Caspase-3 (Asp175) Antibody.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 22 Expand Image
    Confocal immunofluorescent analysis of brain sections from 13-month old wild-type (left) and Tg2576 (center) mice using β-Amyloid (D3D2N) Mouse mAb (green) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (red). A detailed view of a cortical β-amyloid plaque is shown on the right. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 23 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse colon (myenteric plexus) using NeuN (D4G4O) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 24 Expand Image
    Immunoprecipitation of GFAP protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656, and lane 3 is GFAP (E6N9L) Mouse mAb. Western blot analysis was performed GFAP (E6N9L) Mouse mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 25 Expand Image
    Immunohistochemical analysis of paraffin-embedded LL2 syngeneic tumor using HS1 (D5A9) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 26 Expand Image
    Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 27 Expand Image
    Immunoprecipitation of Cleaved PARP (Asp214) from Neuro-2a cell extracts treated with Staurosporine #9953 (1 μM, 3 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cleaved PARP (Asp214) (D6X6X) Rabbit mAb. Western blot was perform using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 28 Expand Image
    Immunohistochemical analysis using Cleaved caspase-3 (Asp175) antibody on SignalSlide™ Cleaved Caspase-3 IHC controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 29 Expand Image
    Immunohistochemical analysis of paraffin-embedded human glioblastoma multiform using NeuN (D4G4O) XP® Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 30 Expand Image
    Confocal immunofluorescent analysis of mouse Tg2576 brain, which overexpresses mutant human APP695. Sections were labeled with GFAP (E6N9L) Mouse mAb (green, mouse IgG2a), β-Amyloid (D3D2N) Mouse mAb #15126 (red, mouse IgG1), and HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) #3892 (magenta, rabbit IgG). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 31 Expand Image
    Confocal immunofluorescent analysis of fixed frozen mouse cortex from wild-type (left) or an amyloid mouse model of Alzheimer's disease (right) using HS1 (D5A9) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 32 Expand Image
    Immunohistochemical analysis of paraffin-embedded 3T3 cell pellet, untreated (left, negative) or treated with Staurosporine #9953 (right, positive), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 33 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 34 Expand Image
    Confocal immunofluorescent analysis of mouse hippocampus (left), cortex (middle), and cerebellum (right) using NeuN (D4G4O) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 35 Expand Image
    Confocal immunofluorescent analysis of mouse Tg2576 brain which overexpresses mutant human APP695. Sections were first labeled with HS1 (D5A9) XP® Rabbit mAb (green) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (yellow). After blocking free secondary binding sites with Mouse (G3A1) mAb IgG1 Isotype Control #5415, sections were incubated with GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (red). Nuclei were labeled with Hoechst 33342 #4082 (blue).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 36 Expand Image
    Confocal immunofluorescent analysis of mouse subicular cortex from an amyloid mouse model of Alzheimer's Disease using β-Amyloid (D54D2) XP® Rabbit mAb #8243 (green) and GFAP (GA5) Mouse mAb #3670 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 37 Expand Image
    Immunohistochemical analysis of paraffin-embedded E14 rat embryo using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 38 Expand Image
    Confocal immunofluorescent analysis of U-251 MG cells (left, positive) or HeLa cells (right, negative) using GFAP (E6N9L) Mouse mAb #34001 (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 39 Expand Image
    Confocal immunofluorescent analysis of 32D cells (left) and C2C12 cells (right), using HS1 (D5A9) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 40 Expand Image
    Immunohistochemical analysis of paraffin-embedded H-4-II-E cell pellet, untreated (left, negative) or treated with Staurosporine #9953 (right, positive), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 41 Expand Image
    Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right), labeled with Cleaved Caspase-3 (Asp175) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin #8953 (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 42 Expand Image
    Flow cytometric analysis of NIH/3T3 cells (blue, negative) and 32D clone 3 cells (green, positive) using HS1 (D5A9) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 43 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse ovary using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 44 Expand Image
    Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3 (Asp175) Antibody compared to a nonspecific negative control antibody (red).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 45 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse spleen using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 46 Expand Image
    Confocal immunofluorescent analysis of Neuro-2a cells, untreated (left, negative) or treated with Staurosporine #9953 (1 μM, 3 hr; right, positive), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 47 Expand Image
    Flow cytometric analysis of serum-starved Neuro-2a cells, untreated (blue) or treated with Staurosporine #9953 (1 μM, 3 hr; green), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    β-Amyloid Mouse Model Neuronal Viability IF Antibody Sampler Kit: Image 48 Expand Image
    Flow cytometric analysis of serum-starved H-4-II-E cells, untreated (blue) or treated with Staurosporine #9953 (1 μM, 3 hr; green), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.