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43159
C1QB (E3U6X) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

C1QB (E3U6X) Rabbit mAb (BSA and Azide Free) #43159

Citations (0)
Filter:
  1. WB
  2. IHC
Western blot analysis of extracts from J774A.1, NIH/3T3, and Neuro-2a cells using C1QB (E3U6X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of C1QB protein in NIH/3T3 and Neuro-2a cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using C1QB (E3U6X) Rabbit mAb performed on the Leica BOND RX. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse liver using C1QB (E3U6X) Rabbit mAb performed on the Leica BOND RX. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse lung using C1QB (E3U6X) Rabbit mAb performed on the Leica BOND RX. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using C1QB (E3U6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse thymus using C1QB (E3U6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse ovary using C1QB (E3U6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse brain using C1QB (E3U6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse spleen using C1QB (E3U6X) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse spleen using C1QB (E3U6X) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded J774A.1 cell pellet (left, positive) or Neuro-2a cell pellet (right, negative) using C1QB (E3U6X) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 43159
Cat. # Size Qty. Price Inventory
43159SF
100 µg

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 30
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #30651. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #30651

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

C1QB (E3U6X) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total C1QB protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse C1QB protein.

Background

C1q is a key subcomponent of the classical complement activation pathway during the innate immune response, and it provides a link to the adaptive immune response (1), as the best-known ligands for C1q are the Fc regions of aggregated IgG and IgM molecules in immune complexes (2). Functional C1q is a complex heterotrimer consisting of the products of three genes: C1QA, C1QB, and C1QC (2). C1q is involved in autoimmunity, infection, inflammatory diseases, and tumors (3). C1q is constitutively synthesized by monocytes, macrophages, dendritic cells (DCs), and microglia, and decreased C1q levels are present in several autoimmune conditions (3,4).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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