Revision 7
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-P, IF-IC

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

130

Source/Isotype:

Mouse IgG1

UniProt ID:

#P16284

Entrez-Gene Id:

5175

Product Information

Product Usage Information

This product is the carrier free version of product #3528. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #3528.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) detects endogenous levels of total CD31 protein. It does not cross-react with other related proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.

Background

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).

  1. Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
  2. Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72.
  3. Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 7
#85873

CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free)

Western Blotting Image 1: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from HUVEC cells using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human smooth muscle using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded HUVEC pellets using CD31 (PECAM-1) (89C2) Mouse mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD31 (PECAM-1) (89C2) Mouse mAb (BSA and Azide Free) Expand Image
Confocal immunofluorescent analysis of HUVEC (left, positive) and HeLa cells (right, negative) using CD31 (PECAM-1) (89C2) Mouse mAb (green), DyLight 554 Phalloidin #13054 (red), and DRAQ5® #4084 (blue). Data were generated using the standard formulation of this product.