Render Target: SSR
Render Timestamp: 2024-12-10T20:07:45.787Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-05-10 22:31:17.416
Product last modified at: 2024-11-26T19:30:11.448Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD31 (PECAM-1) (89C2) Mouse mAb #3528

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:800 - 1:3200
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #85873.

    Protocol

    Specificity / Sensitivity

    CD31 (PECAM-1) (89C2) Mouse mAb detects endogenous levels of total CD31 protein. It does not cross-react with other related proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.

    Background

    CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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