Revision 10

#71810Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, W-S, IP, IHC-P

REACTIVITY:

H M R

SENSITIVITY:

Endogenous

MW (kDa):

55

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q96SW2

Entrez-Gene Id:

51185

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #46351.

Specificity / Sensitivity

CRBN (D8H3S) Rabbit mAb recognizes endogenous levels of total CRBN protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro44 of human CRBN protein.

Background

CRBN/Cereblon, a substrate receptor for the CRL4 E3 ubiquitin ligase, was originally identified for its role in autosomal recessive nonsyndromic mental retardation (1). CRBN’s role in the regulation of the AMPK pathway has been linked to various human diseases, including cardiovascular disease and obesity, and its involvement in proteasomal degradation may be important in neuropsychiatric diseases (2). CRBN has also been shown to enhance the effects of immunomodulatory drugs (IMiDs) in multiple myeloma, and may be a predictive marker for therapy (2-4).

  1. Higgins, J.J. et al. (2004) Neurology 63, 1927-31.
  2. Shi, Q. and Chen, L. (2017) J Immunol Res 2017, 9130608.
  3. Ito, T. and Handa, H. (2016) Int J Hematol 104, 293-9.
  4. Chang, X. et al. (2017) Int J Mol Sci 18, 1999. doi: 10.3390/ijms18091999.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 10
#71810

CRBN (D8H3S) Rabbit mAb

Western Blotting Image 1: CRBN (D8H3S) Rabbit mAb Expand Image
Western blot analysis of extracts from various cell lines using CRBN (D8H3S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: CRBN (D8H3S) Rabbit mAb Expand Image
Simple Western™ analysis of lysates (1 mg/mL) from 3T3 cells using CRBN (D8H3S) Rabbit mAb #71810. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation Image 1: CRBN (D8H3S) Rabbit mAb Expand Image
Immunoprecipitation of CRBN protein from TF-1 extracts. Lane 1 is CRBN (D8H3S) Rabbit mAb #71810, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control#3900, and lane 3 is 10% input. Western blot analysis was performed using CRBN (D8H3S) Rabbit mAb #71810. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody.
Immunohistochemistry Image 1: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 2: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using CRBN (D8H3S) Rabbit mAb
Immunohistochemistry Image 3: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the salivary gland using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 4: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CRBN D8H3S) Rabbit mAb.
Immunohistochemistry Image 5: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 6: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human colon using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 7: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human spleen using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 8: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human testis using CRBN D8H3S) Rabbit mAb.
Immunohistochemistry Image 9: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 10: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 11: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 12: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human normal testis (left), prostate adenocarcinoma (middle), and colon carcinoma (right) using CRBN (D8H3S) Rabbit mAb (top) or a CRBN rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on CRBN protein. The similar staining patterns obtained with both antibodies to help to confirm the specificity of the staining.
Immunohistochemistry Image 13: CRBN (D8H3S) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CRBN (D8H3S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).