Revision 3

#81754Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-P, IF-F, IF-IC

REACTIVITY:

M R

SENSITIVITY:

Endogenous

MW (kDa):

55

Source/Isotype:

Rabbit IgG

UniProt ID:

#P20248

Entrez-Gene Id:

890

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:300 - 1:1200
Immunofluorescence (Frozen) 1:200 - 1:400
Immunofluorescence (Immunocytochemistry) 1:3200 - 1:6400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #18676.

Specificity / Sensitivity

Cyclin A2 (E9Q5G) Rabbit mAb recognizes endogenous levels of total cyclin A2 protein.

Species Reactivity:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human cyclin A2 protein.

Background

While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of overexpressed cyclin A to accelerate the G1 to S transition, causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for the completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).

  1. Pagano, M. et al. (1992) EMBO. J. 11, 961-71.
  2. Resnitzky, D. et al. (1995) Mol. Cell. Biol. 15, 4347-52.
  3. d'Urso, G. et al. (1990) Science 250, 786-91.
  4. Zindy, F. et al. (1992) Biochem. Biophys. Res. Commun. 182, 1144-54.
  5. Furuno, N. et al. (1999) J. Cell. Biol. 147, 295-306.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 3
#81754

Cyclin A2 (E9Q5G) Rabbit mAb

Western Blotting Image 1: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Western blot analysis of extracts from A20, MBT2, and CTLL-2 cells using Cyclin A2 (E9Q5G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of cyclin A2 protein in CTLL-2 cells is consistent with the predicted expression pattern.
Western Blotting Image 2: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse cyclin A2 (mCCNA2-Myc/DDK; +), using Cyclin A2 (E9Q5G) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunohistochemistry Image 1: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using Cyclin A2 (E9Q5G) Rabbit mAb.

Immunohistochemistry Image 2: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using Cyclin A2 (E9Q5G) Rabbit mAb.
Immunohistochemistry Image 3: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using Cyclin A2 (E9Q5G) Rabbit mAb.
Immunohistochemistry Image 4: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded mouse thymus using Cyclin A2 (E9Q5G) Rabbit mAb.
Immunohistochemistry Image 5: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using Cyclin A2 (E9Q5G) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 6: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded MBT2 cell pellet (left, positive) or CTLL-2 cell pellet (right, negative) using Cyclin A2 (E9Q5G) Rabbit mAb.
Immunofluorescence Image 1: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
 
Confocal immunofluorescent analysis of fixed frozen mouse thymus using Cyclin A2 (E9Q5G) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 647 Conjugate) #17846 (right, red) and DAPI #4083 (right, blue).
Immunofluorescence Image 2: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse colon using Cyclin A2 (E9Q5G) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 594 Conjugate) #33908 (right, red) and DAPI #4083 (right, blue).
Immunofluorescence Image 1: Cyclin A2 (E9Q5G) Rabbit mAb Expand Image
Confocal immunofluorescent analysis of NIH/3T3 cells using Cyclin A2 (E9Q5G) Rabbit mAb (left, green), DyLight 554 Phalloidin #13054 (right, red), and DAPI #4083 (right, blue).