Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-Bond, IHC-P, IF-F, IF-IC

REACTIVITY:

H M

SENSITIVITY:

Endogenous

MW (kDa):

12

Source/Isotype:

Rabbit IgG

UniProt ID:

#P30273

Entrez-Gene Id:

2207

Product Information

Product Usage Information

This product is the carrier free version of product #78401. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #78401

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total FcRγ protein. Non-specific staining was observed in normal human kidney and salivary gland by immunohistochemistry.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FcRγ protein.

Background

FcRγ (Fc receptor gamma-chain, FcεRI gamma, High affinity immunoglobulin epsilon receptor subunit gamma) is a component of the IgE Fc receptor FcεRI, a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains (FcRγ) (1,2). FcεRI is expressed exclusively on basophils and mast cells and is involved in triggering allergic reactions (1-3). FcRγ also forms signaling complexes with other Fc receptors, including FcγRIA and FcγRIIIA. FcRγ shows high homology to the T cell receptor (TCR) ζ chain (4-6) and contains an immunoreceptor tyrosine-based activation motif (ITAM) which elicits a pro-inflammatory response (1-3).

  1. Metzger, H. et al. (1986) Annu Rev Immunol 4, 419-70.
  2. Blank, U. et al. (1989) Nature 337, 187-9.
  3. Ra, C. et al. (1989) J Biol Chem 264, 15323-7.
  4. Ra, C. et al. (1989) Nature 341, 752-4.
  5. Küster, H. et al. (1990) J Biol Chem 265, 6448-52.
  6. van Vugt, M.J. et al. (1996) Blood 87, 3593-9.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 1
#98492

FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free)

Western Blotting Image 1: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from various cell lines using FcRγ (E6Y1A) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human neuroendocrine lung carcinoma using FcRγ (E6Y1A) Rabbit mAb
performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human lung sarcoma using FcRγ (E6Y1A) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoblastic lymphoma using FcRγ (E6Y1A) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using FcRγ (E6Y1A) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human salivary small cell carcinoma using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human spleen using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human skin using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human colon using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human brain using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human appendix using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 10: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 11: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse thymus using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 12: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse stomach using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 13: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse lung using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 14: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse liver using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 15: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 16: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FcRγ (E6Y1A) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 17: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or 293T cell pellet (right, negative) using FcRγ (E6Y1A) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with FcRγ (E6Y1A) Rabbit mAb (left, green), and colabeled with DyLight 650 Phalloidin #12956 (right, red) and DAPI #4083 (right, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 2: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse small intestine labeled with FcRγ (E6Y1A) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with LAMP1 (E5N9Z) Rabbit mAb (Alexa Fluor® 594 Conjugate) #73294 (right, yellow pseudocolor), DyLight 650 Phalloidin #12956 (right, red), and DAPI #4083 (right, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: FcRγ (E6Y1A) Rabbit mAb (BSA and Azide Free) Expand Image
Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (left, positive) and 293T cells (right, negative) using FcRγ (E6Y1A) Rabbit mAb (green), DyLight 650 Phalloidin #12956 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.