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48510
Functional Neuron Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Functional Neuron Marker Antibody Sampler Kit #48510

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Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human 5-HTR4 (h5-HTR4-Myc/DDK; +), using 5-HTR4 (D8O5K) Rabbit mAb.
Western blot analysis of extracts from human spinal cord, mouse spinal cord, and mouse kidney using ChAT (E4F9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from mouse and rat tissue using GAD1 (D1F2M) Rabbit mAb (upper) and B-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum using VGLUT1 (E8L5B) Rabbit mAb (left, green), β3-Tubulin (E9F3E) Mouse mAb #45058 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Western blot analysis of extracts from various tissues using VGLUT1 (E8L5B) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of VGLUT1 protein in rat lung tissue is consistent with the predicted expression pattern.
Immunohistochemical analysis of paraffin-embedded mouse olfactory bulb using VGLUT1 (E8L5B) Rabbit mAb.
Western blot analysis of extracts from neonatal mouse and fetal rat brain using GAD2 (D5G2) XP® Rabbit mAb.
Western blot analysis of extracts from various tissues and cell lines using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from mouse brain, mouse lung, rat brain, and human cortex using VGLUT2 (D7D2H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of ACHN cells or 293T cells, either mock transfected (-) or transfected (+) with a cDNA expression construct encoding human β2-adrenergic receptor (hβ2AR), using β2-Adrenergic Receptor (D6H2) Rabbit mAb.
Western blot analysis of extracts from NIH/3T3 and PC-12 cells using 5-HTR4 (D8O5K) Rabbit mAb.
Immunoprecipitation of GAD1 protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is GAD1 (D1F2M) Rabbit mAb. Western blot analysis was performed using GAD1 (D1F2M) Rabbit mAb.
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus using VGLUT1 (E8L5B) Rabbit mAb (left, green), β3-Tubulin (E9F3E) Mouse mAb #45058 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunohistochemical analysis of paraffin-embedded mouse cortex using VGLUT1 (E8L5B) Rabbit mAb.
Confocal immunofluorescent analysis of mouse brain using GAD2 (D5G2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA marker).
Immunohistochemical analysis of paraffin-embedded mouse brain using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunoprecipitation of VGLUT2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VGLUT2 (D7D2H) Rabbit mAb. Western blot analysis was performed using VGLUT2 (D7D2H) Rabbit mAb.
Confocal immunofluorescent analysis of rat retina (left) and cerebellum (right) using GAD1 (D1F2M) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded mouse eye using VGLUT1 (E8L5B) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded PC-12 cell pellet (left, positive) or KNRK cell pellet (right, negative) using Tyrosine Hydroxylase (E2L6M) Rabbit mAb
Immunohistochemical analysis of various paraffin-embedded normal human tissues: brain (left-top), tonsil (middle-top), small intestine (right-top), skin (left-bottom), testis (middle-bottom), and spleen (right-bottom) using VGLUT1 (E8L5B) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Tyrosine Hydroxylase (E2L6M) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human brain (left), mouse cerebellum (middle), or mouse dentate gyrus (right) using VGLUT1 (E8L5B) Rabbit mAb (top) or VGLUT1 Antibody (bottom). These two antibodies detect unique, non-overlapping epitopes on VGLUT1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. 
Immunohistochemical analysis of paraffin-embedded rat small intestine using Tyrosine Hydroxylase (E2L6M) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using VGLUT1 (E8L5B) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded mouse brain using Tyrosine Hydroxylase (E2L6M) Rabbit mAb performed on the Leica BOND Rx.
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or mouse VGLUT1 transfected (right), using VGLUT1 (E8L5B) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse colon using Tyrosine Hydroxylase (E2L6M) Rabbit mAb performed on the Leica BOND Rx.
Confocal immunofluorescent analysis of rat striatum using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of HCC1419 cells (left, positive) and ACHN cells (right, negative) using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Blue = Hoechst 33342 #4082 (fluorescent DNA dye).
Flow cytometric analysis of KNRK cells (blue, negative) and PC-12 cells (green, positive) using Tyrosine Hydroxylase (E2L6M) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 48510
Cat. # Size Qty. Price Inventory
48510T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Tyrosine Hydroxylase (E2L6M) Rabbit mAb 58844 20 µl
  • WB
  • IHC
  • IF
  • F
H M R 55-60 Rabbit IgG
GAD2 (D5G2) XP® Rabbit mAb 5843 20 µl
  • WB
  • IP
  • IF
H M R 60 Rabbit IgG
VGLUT2 (D7D2H) Rabbit mAb 71555 20 µl
  • WB
  • IP
H M R 65-70 Rabbit IgG
VGLUT1 (E8L5B) Rabbit mAb 47181 20 µl
  • WB
  • IHC
  • IF
H M R 62 Rabbit IgG
ChAT (E4F9G) Rabbit mAb 27269 20 µl
  • WB
H M R 71 Rabbit IgG
GAD1 (D1F2M) Rabbit mAb 41318 20 µl
  • WB
  • IP
  • IF
M R 67 Rabbit IgG
5-HTR4 (D8O5K) Rabbit mAb 13690 20 µl
  • WB
H M R 40-140 Rabbit IgG
β2-Adrenergic Receptor (D6H2) Rabbit mAb 8513 20 µl
  • WB
H 50-100 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Functional Neuron Marker Antibody Sampler Kit provides an economical means of detecting markers that facilitate phenotyping neurons by function. The kit includes enough antibodies to perform at least two western blot experiments with each primary antibody.

Background

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines, and acts as a marker for dopaminergic neurons (1,2). Choline O-acetyltransferase (ChAT) is the enzyme that catalyzes synthesis of acetylcholine (ACh) in the central and peripheral nervous system. ChAT is found in high levels within cholinergic neurons and can be used to measure their functional states (3). Vesicular glutamate transporters 1 and 2 (VGLUT1 and VGLUT2) are responsible for transporting the excitatory neurotransmitter glutamate into synaptic vesicles of glutamatergic neurons. VGLUT1 and VGLUT2 are complimentarily expressed and act as markers for glutamatergic neurons (4). Glutamate decarboxylase (GAD) is the main enzyme that synthesizes GABA from glutamate. GABA producing neurons, called GABAergic neurons, utilize GABA as their major inhibitory neurotransmitter with both isoforms of GAD, GAD1, and GAD2, acting as functional markers for these neurons (5). β2-adrenergic receptor (β2AR) is a G protein-coupled receptor (GPCR) that mediates the actions of catecholamines, mainly through stimulation by epinephrine (adrenaline), in the central and peripheral nervous system (6,7). Serotonin receptor 4 (5-HTR4) is an excitatory GPCR that activates the cyclic AMP (cAMP)-PKA pathway (8,9). 5-HTR4 is located post-synaptically on serotonergic neurons (10).

Pathways

Explore pathways related to this product.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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