Cat. # | Size | Qty. | Price | Inventory |
---|---|---|---|---|
5887T | 1 Kit (4 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Nicastrin (D38F9) Rabbit mAb 5665 | 20 µl |
|
H M R Hm Mk | 110, 120 | Rabbit IgG |
PEN2 (D6G8) Rabbit mAb 8598 | 20 µl |
|
H M R Mk | 13 | Rabbit IgG |
Presenilin 1 (D39D1) Rabbit mAb 5643 | 20 µl |
|
H M R Mk | 22 (CTF) | Rabbit IgG |
Presenilin 2 (D30G3) Rabbit mAb 9979 | 20 µl |
|
H M R | 23 (CTF) | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Rab | Goat |
Product Information
Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Presenilin 1 protein, a synthetic peptide corresponding to residues surrounding Val390 of human Nicastrin protein, a synthetic peptide corresponding to residues surrounding Leu92 of human PEN2 protein, or a synthetic peptide corresponding to residues surrounding Met323 of human Presenilin 2 protein.
The γ secretase protease complex interacts with and cleaves intramembrane substrates as an essential function for regulation of intracellular signaling and cell-cell interactions. This multiprotein complex is comprised of four integral membrane proteins, Presenilin, Nicastrin, Aph-1, and PEN2, all of which are essential for complete proteolytic activity (1). Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin’s associated γ-secretase activity for amyloid-β protein processing (2,3). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (3,4). Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (5,6). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (7). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to γ-secretase to ensure the correct cleavage process (6,8). Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (9). PEN2 is an important part of the γ-secretase complex as its knock down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (10).
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