Revision 1

#24642Store at -20C

1 Kit

(5 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
GATA-1 (D52H6) XP® Rabbit mAb 3535 20 µl 43 kDa Rabbit IgG
GATA-2 (E9T6F) Rabbit mAb 79802 20 µl 51 kDa Rabbit IgG
GATA-3 (D13C9) XP® Rabbit mAb 5852 20 µl 48 kDa Rabbit IgG
GATA-4 (D3A3M) Rabbit mAb 36966 20 µl 55 kDa Rabbit IgG
GATA-6 (D61E4) XP® Rabbit mAb 5851 20 µl 55 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The GATA Transcription Factor Antibody Sampler Kit provides an economical means of evaluating total levels of GATA family proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). Although they are commonly divided as hematopoietic (GATA-1-3) or cardiac (GATA-4-6) factors, GATA proteins are expressed in a wide variety of tissue and play critical roles in embryonic development and organ differentiation (4). GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (5,6). Mutations in the corresponding GATA-1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (7-10). GATA-2 is widely expressed and plays an essential role in many developmental processes (11). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (12). GATA-2 also inhibits the differentiation of white and brown adipocytes and has been shown to suppress the proliferation of neuronal progenitor cells (13-15). GATA-3 is a critical regulator of development and is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (16-19). GATA-3 knockout mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (20). The function of GATA-3 has also been extensively studied in T cell development and has been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (21,22). GATA-4 is crucial for cardiomyocyte differentiation, and not surprisingly, mutations in the GATA-4 gene are implicated in many cardiac diseases, such as tetralogy of Fallot, familial and sporadic dilated cardiomyopathy, and atrial septal defect (23-27). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (28). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (29). GATA-6 plays a critical role in endoderm development and is essential for the development of the heart, gut, and other organs (30-32). Knockout of GATA-6 is embryonic lethal due to defects in the formation of the heart tube and a failure to develop extraembryonic endoderm (30).
 

  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Tremblay, M. et al. (2018) Development 145, dev164384. doi: 10.1242/dev.164384.
  5. Pevny, L. et al. (1991) Nature 349, 257-60.
  6. Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
  7. Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
  8. Cantor, A.B. (2005) Int J Hematol 81, 378-84.
  9. Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
  10. Tubman, V.N. et al. (2007) Blood 109, 3297-9.
  11. Tong, Q. et al. (2003) Drug News Perspect 16, 585-8.
  12. Tsai, F.Y. et al. (1994) Nature 371, 221-6.
  13. Tong, Q. et al. (2005) Mol Cell Biol 25, 706-15.
  14. Tsai, J. et al. (2005) EMBO Rep 6, 879-84.
  15. El Wakil, A. et al. (2006) Development 133, 2155-65.
  16. Debacker, C. et al. (1999) Mech Dev 85, 183-7.
  17. Grote, D. et al. (2008) PLoS Genet 4, e1000316.
  18. Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
  19. Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
  20. Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
  21. Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
  22. Amsen, D. et al. (2007) Immunity 27, 89-99.
  23. Gan, L. et al. (2014) Gene Expr Patterns 16, 8-22.
  24. Yang, Y.Q. et al. (2013) Hum Mutat 34, 1662-71.
  25. Li, R.G. et al. (2013) Biochem Biophys Res Commun 439, 591-6.
  26. Li, J. et al. (2014) Gene 548, 174-81.
  27. Mohan, R.A. et al. (2014) Am J Med Genet A 164A, 2732-8.
  28. Walker, E.M. et al. (2014) Dev Biol 392, 283-94.
  29. LaVoie, H.A. (2014) Biol Reprod 91, 38.
  30. Cai, K.Q. et al. (2008) Dev Dyn 237, 2820-9.
  31. Charron, F. and Nemer, M. (1999) Semin Cell Dev Biol 10, 85-91.
  32. Haveri, H. et al. (2008) BMC Gastroenterol 8, 9.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Revision 1
    #24642

    GATA Transcription Factor Antibody Sampler Kit

    GATA Transcription Factor Antibody Sampler Kit: Image 1 Expand Image
    Flow cytometric analysis of THP-1 cells (blue, negative) and MCF7 cells (green, positive) using GATA-3 (D13C9) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    GATA Transcription Factor Antibody Sampler Kit: Image 2 Expand Image
    Western blot analysis of lysates from various cell lines using GATA-1 (D52H6) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 3 Expand Image
    Western blot analysis of extracts from various cell lines using GATA-4 (D3A3M) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells exhibit very low GATA-4 expression.
    GATA Transcription Factor Antibody Sampler Kit: Image 4 Expand Image
    Western blot analysis of extracts from Huh7 and 293 cells using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 5 Expand Image
    CUT&Tag was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across HNMT, a known target gene of GATA-6 (see our CUT&RUN-qPCR figure).
    GATA Transcription Factor Antibody Sampler Kit: Image 6 Expand Image
    Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 7 Expand Image
    Western blot analysis of extracts from CEM and SH-SY5Y cells using GATA-3 (D13C9) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 8 Expand Image
    CUT&RUN was performed with NK-92 cells and either GATA-3 (D13C9) XP® Rabbit mAb or GATA-3 (E2N1Y) Mouse mAb #96098, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across SH3RF3, a known target gene of GATA-3 (see additional figure containing CUT&RUN-qPCR data).
    GATA Transcription Factor Antibody Sampler Kit: Image 9 Expand Image
    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
    GATA Transcription Factor Antibody Sampler Kit: Image 10 Expand Image
    Immunoprecipitation of GATA-2 protein from SH-SY5Y cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is GATA-2 (E9T6F) Rabbit mAb. Western blot analysis was performed using GATA-2 (E9T6F) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
    GATA Transcription Factor Antibody Sampler Kit: Image 11 Expand Image
    Western blot analysis of extracts from various cell lines using GATA-2 (E9T6F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of GATA-2 protein in U-2 OS and Jurkat cells is consistent with the predicted expression pattern and published observations.
    GATA Transcription Factor Antibody Sampler Kit: Image 12 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either GATA-2 (E9T6F) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human GATA2 intron 4 primers, human RAP1GAP intron 2 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    GATA Transcription Factor Antibody Sampler Kit: Image 13 Expand Image
    Flow cytometric analysis of Jurkat cells (blue, negative) and CMK cells (green, positive) using GATA-2 (E9T6F) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    GATA Transcription Factor Antibody Sampler Kit: Image 14 Expand Image
    Confocal immunofluorescent analysis of SH-SY5Y cells (left, positive) and U-2 OS cells (right, negative) using GATA-2 (E9T6F) Rabbit mAb (green), DyLight 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
    GATA Transcription Factor Antibody Sampler Kit: Image 15 Expand Image
    CUT&RUN was performed with K562 cells and either GATA-2 (E9T6F) Rabbit mAb or GATA-2 (E8B5C) Rabbit mAb #72445, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across EIF4E, a known target gene of GATA-2 (see additional figure containing CUT&RUN-qPCR data).
    GATA Transcription Factor Antibody Sampler Kit: Image 16 Expand Image
    Immunohistochemical analysis of paraffin-embedded human bone marrow using GATA-1 (D52H6) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 17 Expand Image
    Confocal immunofluorescent analysis of Huh7 (left) and HeLa (right) cells using GATA-4 (D3A3M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).
    GATA Transcription Factor Antibody Sampler Kit: Image 18 Expand Image
    Confocal immunofluorescent analysis of KM12 (left) and SK-OV-3 cells (right) using GATA-6 (D61E4) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
    GATA Transcription Factor Antibody Sampler Kit: Image 19 Expand Image
    CUT&Tag was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across chromosome 2 (upper), including HNMT (lower), a known target gene of GATA-6 (see our CUT&RUN-qPCR figure).
    GATA Transcription Factor Antibody Sampler Kit: Image 20 Expand Image
    Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 21 Expand Image
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GATA-3 (D13C9) XP® Rabbit mAb (left) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
    GATA Transcription Factor Antibody Sampler Kit: Image 22 Expand Image
    CUT&RUN was performed with NK-92 cells and either GATA-3 (D13C9) XP® Rabbit mAb or GATA-3 (E2N1Y) Mouse mAb #96098, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figures show binding across chromosome 2 (upper), including SH3RF3 (lower), a known target gene of GATA-3 (see additional figure containing CUT&RUN-qPCR data).
    GATA Transcription Factor Antibody Sampler Kit: Image 23 Expand Image
    Western blot analysis of extracts from CMK and PC-12 cells using GATA-2 (E9T6F) Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 24 Expand Image
    CUT&RUN was performed with K562 cells and either GATA-2 (E9T6F) Rabbit mAb or GATA-2 (E8B5C) Rabbit mAb #72445, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 4 (upper), including EIF4E (lower), a known target gene of GATA-2 (see additional figure containing CUT&RUN-qPCR data).
    GATA Transcription Factor Antibody Sampler Kit: Image 25 Expand Image
    Immunohistochemical analysis of formalin-fixed paraffin-embedded undecalcified mouse bone using GATA-1 (D52H6) XP® Rabbit mAb. Note staining of cells in the marrow.
    GATA Transcription Factor Antibody Sampler Kit: Image 26 Expand Image
    Flow cytometric analysis of HeLa (blue) and ATL-16T cells (green) using GATA-4 (D3A3M) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    GATA Transcription Factor Antibody Sampler Kit: Image 27 Expand Image
    Flow cytometric analysis of Jurkat cells (blue, negative) and BT-549 cells (green, positive) using GATA-6 (D61E4) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    GATA Transcription Factor Antibody Sampler Kit: Image 28 Expand Image
    Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 29 Expand Image
    Immunohistochemical analysis of paraffin-embedded cell pellets, MCF-7 (left) or HUVEC (right), using GATA-3 (D13C9) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 30 Expand Image
    CUT&RUN was performed with NK-92 cells and either GATA-3 (D13C9) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human RBM15 promoter primers, human SH3RF3 intron 1 primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    GATA Transcription Factor Antibody Sampler Kit: Image 31 Expand Image
    Western blot analysis of extracts from BA/F3 cells using GATA-2 (E9T6F) Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 32 Expand Image
    CUT&RUN was performed with K562 cells and either GATA-2 (E9T6F) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human EIF4E promoter primers, human COX14 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    GATA Transcription Factor Antibody Sampler Kit: Image 33 Expand Image
    Confocal immunofluorescent analysis of K-562 (left) and HeLa (right) cells using GATA-1 (D52H6) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    GATA Transcription Factor Antibody Sampler Kit: Image 34 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across FGFR2, a known target gene of GATA-6 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.
    GATA Transcription Factor Antibody Sampler Kit: Image 35 Expand Image
    Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 36 Expand Image
    Confocal Immunofluorescent analysis of MCF7 (left) and HUVE (right) cells using GATA-3 (D13C9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
    GATA Transcription Factor Antibody Sampler Kit: Image 37 Expand Image
    Flow cytometric analysis of HeLa cells (red) and K-562 cells (blue) using GATA-1 (D52H6) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 38 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across chromosome 10 (upper), including FGFR2 (lower), a known target gene of GATA-6 (see additional figure containing ChIP-qPCR data).
    GATA Transcription Factor Antibody Sampler Kit: Image 39 Expand Image
    Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 40 Expand Image
    Flow cytometric analysis of THP-1 cells (blue) and MCF7 cells (green) using GATA-3 (D13C9) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 41 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Caco-2 cells and either GATA-6 (D61E4) XP® Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human FDPS Promoter Primers #13840, human FGFR2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    GATA Transcription Factor Antibody Sampler Kit: Image 42 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 43 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NK-92 cells and either GATA-3 (D13C9) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCL4 promoter primers, SimpleChIP® Human IFN-γ Promoter Primers #13051, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    GATA Transcription Factor Antibody Sampler Kit: Image 44 Expand Image
    CUT&RUN was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across FGFR2 gene.
    GATA Transcription Factor Antibody Sampler Kit: Image 45 Expand Image
    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 46 Expand Image
    CUT&RUN was performed with Caco-2 cells and GATA-6 (D61E4) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 10 (upper), including FGFR2 gene (lower).
    GATA Transcription Factor Antibody Sampler Kit: Image 47 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human stomach using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 48 Expand Image
    CUT&RUN was performed with Caco-2 cells and either GATA-6 (D61E4) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human HNMT upstream primer, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    GATA Transcription Factor Antibody Sampler Kit: Image 49 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human small intestine using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 50 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human pancreas using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 51 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 52 Expand Image
    >Immunohistochemical analysis of paraffin-embedded normal human lung using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 53 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human heart using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 54 Expand Image
    Immunohistochemical analysis of paraffin-embedded human appendix using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 55 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human adrenal gland using GATA-6 (D61E4) XP ® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 56 Expand Image
    Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 57 Expand Image
    Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 58 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse testis using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 59 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse ovary using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 60 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse colon using GATA-6 (D61E4) XP® Rabbit mAb
    GATA Transcription Factor Antibody Sampler Kit: Image 61 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse small intestine using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 62 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse brain using GATA-6 (D61E4) XP® Rabbit mAb.
    GATA Transcription Factor Antibody Sampler Kit: Image 63 Expand Image
    Immunohistochemical analysis of paraffin-embedded normal human colon using GATA-6 (D61E4) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
    GATA Transcription Factor Antibody Sampler Kit: Image 64 Expand Image

    Immunohistochemical analysis of paraffin-embedded HuH-7 cell pellet (left, positive) and SUP-B15 cell pellet (right, negative) using GATA-6 (D61E4) XP® Rabbit mAb.

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