Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-Bond, IHC-P

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

30-40

Source/Isotype:

Rabbit IgG

UniProt ID:

#P01903

Entrez-Gene Id:

3122

Product Information

Product Usage Information

This product is the carrier free version of product #97971. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #97971.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total HLA-DRA protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val153 of human HLA-DRA protein.

Background

Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).

  1. Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
  2. Cresswell, P. (1994) Annu Rev Immunol 12, 259-93.
  3. Karp, D.R. et al. (1990) J Exp Med 171, 615-28.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 1
#25328

HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free)

Western Blotting Image 1: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing full-length human Myc/DDK-tagged HLA-DRA (hHLA-DRA-Myc/DDK), HLA-DPA1 (hHLA-DPA1-Myc/DDK), HLA-DOA (hHLA-DOA-Myc/DDK), HLA-DQA1 (hHLA-DQA1-MycDDK), HLA-DQA2 (hHLA-DQA2-Myc/DKK), or HLA-DMA (hHLA-DMA-Myc/DKK), using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from various cell lines using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded Raji cell pellet (left, positive) or Jurkat cell pellet (right, negative) using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma (left) or colon carcinoma (right) using HLA-DRA (E9R2Q) XP® Rabbit mAb (top) or HLA-DRA Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human HLA-DRA. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: HLA-DRA (E9R2Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.