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95742
IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (BSA and Azide Free) #95742

Citations (0)
Filter:
  1. WB
  2. IHC
  3. IF
  4. F
Western blot analysis of extracts from various cells using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse thymus using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse colon using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (left) or IL-2Rα/CD25 (E4B2Z) XP® Rabbit mAb #36128 (right). These two antibodies detect independent, unique epitopes on mouse IL-2Rα/CD25. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded CTLL-2 cell pellet (left, positive) or NIH/3T3 cell pellet (right, negative) using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse spleen using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled with CD4 (RM4-5) Rat mAb (FITC Conjugate) #96127 (blue pseudocolor) and FoxP3 (D6O8R) Rabbit mAb (Alexa Fluor® 647 Conjugate) (red). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse spleen using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (green), CD4 (RM4-5) Rat mAb (FITC Conjugate) #96127 (red pseudocolor), and CD45R/B220 (RA3-6B2) Rat mAb (redFluor 710 Conjugate) #82984 (cyan pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse colon using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (green) and CD4 (RM4-5) Rat mAb (FITC Conjugate) #96127 (red pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Note: signal in myenteric plexus is presumed to be non-specific. Data were generated using the standard formulation of this product.
Flow cytometric analysis of live mouse splenocytes using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left), and co-stained with CD4 (RM4-5) Rat mAb (FITC Conjugate) #96127. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. Data were generated using the standard formulation of this product.
Flow cytometric analysis of live C2C12 cells (blue, negative) and CTLL-2 cells (green, positive) using IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 95742
Cat. # Size Qty. Price Inventory
95742SF
100 µg

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 40-80
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #39475. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.



This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #39475.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

IL-2Rα/CD25 (E9W2J) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total IL-2Rα/CD25 protein. This antibody non-specifically labels myenteric nerves in the gut.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IL-2Rα/CD25 protein.

Background

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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