Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-P, FC-FP

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

11

Source/Isotype:

Rabbit IgG

UniProt ID:

#P10145

Entrez-Gene Id:

3576

Product Information

Product Usage Information

This product is the carrier-free version of product #94407. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #94407.

Storage

Store at –20°C. This product will freeze at –20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vorteing. This will not interfere with antibody performance.

Specificity / Sensitivity

IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total IL-8 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human IL-8 recombinant protein.

Background

The prototypical CXC chemokine, IL-8, is best known for effects on neutrophils, specifically its ability to act as a chemoattractant and activate degranulation and respiratory burst (1-3).

IL-8 is produced by a number of cell types, including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct receptors for IL-8, CXCR1 and CXCR2; both are G protein-coupled receptors (1). Ligand binding induces Ca2+ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).

  1. Payne, A.S. and Cornelius, L.A. (2002) J Invest Dermatol 118, 915-22.
  2. Brat, D.J. et al. (2005) Neuro Oncol 7, 122-33.
  3. Mukaida, N. (2003) Am J Physiol Lung Cell Mol Physiol 284, L566-77.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 1
#10681

IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free)

Western Blotting Image 1: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of recombinant Human Interleukin-8 (hIL-8) #94853 using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from U-937 cells, untreated (-) or treated (+) as indicated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/mL, 24 hr), Lipopolysaccharides (LPS) #14011 (5 μg/mL, 7 hr), and/or Brefeldin A #9972 (300 ng/mL, 3 hr), using IL-8 (E5F5Q) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human tonsil using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using IL-8 (E5F5Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma (left) or endometrioid adenocarcinoma (right) using IL-8 (E5F5Q) XP® Rabbit mAb (top) or IL-8 Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human IL-8. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or IL-8-transfected (right), using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded U-937 cell pellet, untreated (left, negative) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/mL, 24 hr), Lipopolysaccharides (LPS) #14011 (5 μg/ml, 7 hr), and Brefeldin A #9972 (300 ng/mL, 3 hr) (right, positive), using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using IL-8 (E5F5Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: IL-8 (E5F5Q) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Flow cytometric analysis of U-937 cells, untreated (blue) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/mL, 24 hr), Lipopolysaccharides (LPS) #14011 (5 μg/mL, 7 hr), and Brefeldin A #9972 (300 ng/mL, 3 hr) (green), using IL-8 (E5F5Q) XP® Rabbit mAb (solid lines) or concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.