Revision 1

#24544Store at -20C

1 Kit

(5 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

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For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Doublecortin (A8L1U) Rabbit mAb 14802 20 µl Rabbit IgG
NeuN (D4G4O) XP® Rabbit mAb 24307 20 µl 46-55 kDa Rabbit IgG
GFAP (GA5) Mouse mAb 3670 20 µl 50 kDa Mouse IgG1
Myelin Basic Protein (D8X4Q) XP® Rabbit mAb 78896 20 µl 12-18 kDa Rabbit IgG
Ki-67 (D3B5) Rabbit mAb 9129 20 µl Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Neuronal Marker IF Antibody Sampler Kit II provides an economical means for labeling cell types and cell structures by immunofluorescence (IF-F).

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The antibodies in this kit serve as markers to determine cell types in the brain. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. Doublecortin is expressed in neuronal precursor cells and expression is downregulated as neurons mature (1). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (2). As Doublecortin is downregulated, NeuN is upregulated (1,2). GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP is commonly used by investigators as a marker for intracranial and intraspinal tumors arising from astrocytes (3). Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes (4). Ki-67 is universally expressed among proliferating cells and absent in quiescent cells. Specifically, it is detected in proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase (5).

  1. Brown, J.P. et al. (2003) J Comp Neurol 467, 1-10.
  2. Mullen, R.J. et al. (1992) Development 116, 201-11.
  3. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
  4. Harauz, G. and Boggs, J.M. (2013) J Neurochem 125, 334-61.
  5. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    使用に関する制限

    法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

    研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

    Revision 1
    #24544

    Neuronal Marker IF Antibody Sampler Kit II

    Neuronal Marker IF Antibody Sampler Kit II: Image 1 Expand Image
    Western blot analysis of extracts from mouse brain, C2C12 cells, and rat brain using NeuN (D4G4O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
    Neuronal Marker IF Antibody Sampler Kit II: Image 2 Expand Image
    Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 3 Expand Image
    Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
    Neuronal Marker IF Antibody Sampler Kit II: Image 4 Expand Image
    Confocal immunofluorescent analysis of the ventricular zone in P21 mouse brain using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DyLight 554 phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 5 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum using NeuN (D4G4O) XP® Rabbit mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 6 Expand Image
    Immunohistochemical analysis of paraffin-embedded cynomolgus monkey brain using NeuN (D4G4O) XP® Rabbit mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 7 Expand Image
    Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using GFAP (GA5) Mouse mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 8 Expand Image
    Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 9 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 10 Expand Image
    Confocal immunofluorescent analysis of Doublecortin (A8L1U) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red) staining in the subventricular zone of adult mouse brain. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 11 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse colon (myenteric plexus) using NeuN (D4G4O) XP® Rabbit mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 12 Expand Image
    Confocal immunofluorescence image of rat hippocampus labeled with GFAP (GA5) Mouse mAb (red), Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green), and CREB (48H2) Rabbit mAb #9197 (blue).
    Neuronal Marker IF Antibody Sampler Kit II: Image 13 Expand Image
    Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
    Neuronal Marker IF Antibody Sampler Kit II: Image 14 Expand Image
    Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Neuronal Marker IF Antibody Sampler Kit II: Image 15 Expand Image
    Confocal immunofluorescent analysis of P5 mouse cerebellum using Doublecortin (A8L1U) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 16 Expand Image
    Immunohistochemical analysis of paraffin-embedded human glioblastoma multiform using NeuN (D4G4O) XP® Rabbit mAb.
    Neuronal Marker IF Antibody Sampler Kit II: Image 17 Expand Image
    Confocal immunofluorescent analysis of SNB19 (positive, left) and HeLa (negative, right) cells, using GFAP (GA5) Mouse mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 18 Expand Image
    Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 19 Expand Image
    Confocal immunofluorescent analysis of mouse hippocampus (left), cortex (middle), and cerebellum (right) using NeuN (D4G4O) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Neuronal Marker IF Antibody Sampler Kit II: Image 20 Expand Image
    Flow cytometric analysis of HeLa cells (blue) and SNB-19 cells (green) using GFAP (GA5) Mouse mAb (solid lines) or a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
    Neuronal Marker IF Antibody Sampler Kit II: Image 21 Expand Image
    Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).