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72007
PhosphoPlus® Paxillin (Tyr118) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® Paxillin (Tyr118) Antibody Duet #72007

Citations (0)
Western blot analysis of extracts from various cell lines using Paxillin (D9G12) Rabbit mAb. The 45 kDa band is a degradation product of full-length paxillin protein.
Western blot analysis of extracts from HeLa cells in suspension, untreated (-) or induced attachment to fibronectin-coated plate surface (10 μg/ml, 1 hr; +), using Phospho-Paxillin (Tyr118) (E9U9F) Rabbit mAb (upper) and Paxillin (D9G12) Rabbit mAb #12065 (lower).
Immunoprecipitation of Phospho-Paxillin (Tyr118) protein from HeLa cells induced attachment to fibronectin-coated plate surface (10 μg/ml, 1 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Paxillin (Tyr118) (E9U9F) Rabbit mAb. Western blot analysis was performed using Phospho-Paxillin (Tyr118) (E9U9F) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
HeLa cells were detached with 0.04% trypsin and 0.03% EDTA, suspended in serum-free medium for one hour, and either processed in suspension (right) or plated on fibronectin-coated slides and allowed to adhere for an additional hour prior to fixation (left). Adherent cells were post-processed with λ-phosphatase to confirm phospho-specificity (center). Confocal immunofluorescent analysis was performed after staining with Phospho-Paxillin (Tyr118) (E9U9F) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
To Purchase # 72007
Cat. # Size Qty. Price Inventory
72007S
1 Kit

Product Includes Quantity Reactivity MW(kDa) Isotype
Paxillin (D9G12) Rabbit mAb 12065 100 µl H 54, 62, 68 Rabbit IgG
Phospho-Paxillin (Tyr118) (E9U9F) Rabbit mAb 69363 100 µl H M R Mk 68 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

Pathways

Explore pathways related to this product.

Limited Uses

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