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38239
PhosphoPlus® PBRM1/BAF180 (Ser948) Antibody Duet
Primary Antibodies
Antibody Duet
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PhosphoPlus® PBRM1/BAF180 (Ser948) Antibody Duet #38239

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Western blot analysis of extracts from HeLa cells, untreated (-), UV-treated (+), or UV-treated and subsequently treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-PBRM1/BAF180 (Ser948) (E6Z2C) Rabbit mAb (upper) or PBRM1/BAF180 (E9X2Z) Rabbit mAb #89123 (lower).
Western blot analysis of extracts from various cell lines using PBRM1/BAF180 (E9X2Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, HCC1143 cells are negative for PBRM1/BAF180 expression.
Confocal immunofluorescent analysis of HeLa cells, untreated (left, low-expressing), UV-irradiated (100 mJ/cm2, 1 hr recovery; middle, high-expressing), or UV-irradiated and post-processed with λ phosphatase (right, negative), using Phospho-PBRM1/BAF180 (Ser948) (E6Z2C) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
Immunoprecipitation of PBRM1/BAF180 protein from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) maAb IgG XP® Isotype Control #3900, and lane 3 is PBRM1/BAF180 (E9X2Z) Rabbit mAb. Western blot analysis was performed using PBRM1/BAF180 (E9X2Z) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 days, then treated with β-estradiol (100 nM) for 45 minutes and either PBRM1/BAF180 (E9X2Z) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using Human ESR1 Intron 1 Primers, SimpleChIP® Human CCND1 Promoter Primers #12531, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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To Purchase # 38239
Cat. # Size Qty. Price Inventory
38239S		 1 Kit

Bulk & Custom Formulation

Product Includes Quantity Reactivity MW(kDa) Isotype
PBRM1/BAF180 (E9X2Z) Rabbit mAb 89123 100 µl H M R Mk 205 Rabbit IgG
Phospho-PBRM1/BAF180 (Ser948) (E6Z2C) Rabbit mAb 82088 100 µl H M 205 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).

PBRM1/BAF180 is a unique member of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin (10,11). PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation (12,13). PBRM1/BAF180 has been shown to be a potent tumor suppressor, as it is the second-most mutated gene in renal carcinomas (14). Mutations of PBRM1/BAF180 have also been shown to be involved in breast cancer, and low expression relates to poorer prognosis (15,16). PBRM1/BAF180 is phosphorylated at Ser948 by ATM during DNA damage, which is important for transcriptional silencing and repair around double-stranded breaks (17).
PBRM1/BAF180 is phosphorylated at Ser948 by ATM during DNA damage, which is important for transcriptional silencing and repair around double-stranded breaks (17).

  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Nie, Z. et al. (2000) Mol Cell Biol 20, 8879-88.
  11. Xue, Y. et al. (2000) Proc Natl Acad Sci U S A 97, 13015-20.
  12. Lemon, B. et al. (2001) Nature 414, 924-8.
  13. Wang, Z. et al. (2004) Genes Dev 18, 3106-16.
  14. Varela, I. et al. (2011) Nature 469, 539-42.
  15. Xia, W. et al. (2008) Cancer Res 68, 1667-74.
  16. Mo, D. et al. (2015) Int J Clin Exp Pathol 8, 9307-13.
  17. Kakarougkas, A. et al. (2014) Mol Cell 55, 723-32.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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