Render Target: SSR
Render Timestamp: 2025-01-30T17:19:33.941Z
Commit: 1bba917eefc12d62e72a522121e2774ffbd0ee36
XML generation date: 2024-04-05 20:52:16.079
Product last modified at: 2025-01-01T09:00:51.180Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-AAK1 (Ser637/Thr640) Antibody #92438

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 142
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-AAK1 (Ser637/Thr640) Antibody recognizes endogenous levels of AAK1 protein only when phosphorylated at Ser637, Thr640, and Ser637/Thr640.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser637/Thr640 of human AAK1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Adaptor-associated kinase 1 (AAK1) is a member of the Ark1/Prk1 family of serine/threonine kinases (1). AAK1 is enriched in synaptosomal preparations and modulates clathrin-dependent endocytosis, a process that is important in synaptic vesicle recycling and receptor-mediated endocytosis. AAK1, together with clathrin, and clathrin-adaptor protein, AP-2, forms a signaling complex at the cell membrane. AAK1-dependent phosphorylation of the mu-2 subunit of AP-2 enhances endocytosis efficiency (2,3). AAK1 is known to promote neuregulin/ErbB4 internalization to regulate neurotrophic signaling. Inhibition of AAK1 activity promotes cell surface expression of neuregulin/ErbB4, cell-bound neurotrophic factors that are implicated in brain development and synaptic plasticity (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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