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| Cat. # | Size | Qty. | Price | Inventory |
|---|---|---|---|---|
| 10396T | 1 Kit (6 x 20 microliters) |
|
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb 4060 | 20 µl |
|
H M R Hm Mk Dm Z B | 60 | Rabbit IgG |
| Akt (pan) (C67E7) Rabbit mAb 4691 | 20 µl |
|
H M R Mk Dm | 60 | Rabbit IgG |
| Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb 9018 | 20 µl |
|
H M R | 60 | Rabbit IgG |
| Akt1 (C73H10) Rabbit mAb 2938 | 20 µl |
|
H M R Mk | 60 | Rabbit IgG |
| Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb 8599 | 20 µl |
|
H M R | 60 | Rabbit IgG |
| Akt2 (D6G4) Rabbit mAb 3063 | 20 µl |
|
H M R Mk | 60 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Rab | Goat |
Product Information
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt. Akt (pan) (C67E7) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of mouse Akt. Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific) is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt1 protein. Akt1 (C73H10) Rabbit mAb is produced by immunizing animals with a synthetic peptide surrounding Leu110 of human Akt1. Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 Specific) is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser474 of human Akt2 protein. Akt2 (D6G4) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues of human Akt2.
Akt, also referred to as PKB or Rac, plays a critical role in controlling cell survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
There are three Akt isoforms (Akt1, Akt2 and Akt3) in mammals (20). Akt activation requires phosphorylation by mTORC2 at Ser473 of Akt1, Ser474 of Akt2, and Ser472 of Akt3 (20).
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