Revision 1

#9788Store at -20C

1 Kit

(6 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
SUZ12 (D39F6) XP® Rabbit mAb 3737 20 µl 83 kDa Rabbit IgG
Ezh2 (D2C9) XP® Rabbit mAb 5246 20 µl 98 kDa Rabbit IgG
Ring1A (D2P4D) Rabbit mAb 13069 20 µl 54 kDa Rabbit IgG
RING1B (D22F2) XP® Rabbit mAb 5694 20 µl 41 kDa Rabbit IgG
Bmi1 (D20B7) XP® Rabbit mAb 6964 20 µl 41, 43 kDa Rabbit IgG
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb 9733 20 µl 17 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Polycomb Group Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group Proteins. The kit contains enough primary and secondary antibodies to perform two western mini-blot experiments.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell-cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, Eed-Ezh2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinates histone H2A on Lys119 (6). PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of an additional catalytic subunit RING1B (also RING2 or RNF2) (7). PcG proteins play an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and are required for maintenance of adult hematopoietic and neural stem cells, as well as embryonic stem cells (3,4,8-10).

  1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
  2. Lee, T.I. et al. (2006) Cell 125, 301-13.
  3. Park, I.K. et al. (2003) Nature 423, 302-5.
  4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
  5. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
  6. Wang, H. et al. (2004) Nature 431, 873-8.
  7. Cao, R. et al. (2005) Mol Cell 20, 845-54.
  8. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
  9. Jacobs, J.J. et al. (1999) Nature 397, 164-8.
  10. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    使用に関する制限

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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Revision 1
    #9788

    Polycomb Group Antibody Sampler Kit

    Polycomb Group Antibody Sampler Kit: Image 1 Expand Image
    Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from K-562 cells and SUZ12 (D39F6) XP® Rabbit mAb using a protocol based on the RBP-eCLIP Kit from EclipseBio. The figure shows binding across the NOTCH1 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.
    Polycomb Group Antibody Sampler Kit: Image 2 Expand Image
    Simple Western™ analysis of lysates (0.1 mg/mL) from MCF-7 cells using Ezh2 (D2C9) XP® Rabbit mAb #5246. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ ​​​​​​​ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66 – 440 kDa separation module.
    Polycomb Group Antibody Sampler Kit: Image 3 Expand Image
    Western blot analysis of extracts from various cell lines using Ring1A (D2P4D) Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 4 Expand Image
    Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 5 Expand Image
    Western blot analysis of extracts from MCF7, Neuro-2a, and COS-7 cell lines using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 6 Expand Image
    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Ezh2 (D2C9) XP® Rabbit mAb performed on the Leica BOND Rx.
    Polycomb Group Antibody Sampler Kit: Image 7 Expand Image
    CUT&Tag was performed with NCCIT cells and Ezh2 (D2C9) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across HOXA1, a known target gene of Ezh2 (see our ChIP-qPCR figure).
    Polycomb Group Antibody Sampler Kit: Image 8 Expand Image
    Western blot analysis of extracts from various cell lines using RING1B (D22F2) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 9 Expand Image
    Western blot analysis of extracts from various cell lines using Bmi1 (D20B7) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 10 Expand Image
    CUT&Tag was performed with NCCIT cells and Bmi1 (D20B7) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across HOXA1, a known target gene of Bmi1 (see our ChIP-qPCR figure).
    Polycomb Group Antibody Sampler Kit: Image 11 Expand Image
    CUT&RUN was performed with NCCIT cells and either Bmi1 (D20B7) XP® Rabbit mAb or CBX4 (E6L7X) Rabbit mAb #30559, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across HoxA1 gene, a known target gene of Bmi1 (see additional figure containing CUT&RUN-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 12 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and Bmi1 (D20B7) XP® Rabbit mAb using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across HoxA, a known target gene of Bmi1 (see additional figure containing ChIP-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 13 Expand Image
    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
    Polycomb Group Antibody Sampler Kit: Image 14 Expand Image
    Western blot analysis of various cell lines using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 15 Expand Image
    Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb performed on the Leica® BOND™ Rx.
    Polycomb Group Antibody Sampler Kit: Image 16 Expand Image
    CUT&Tag was performed with NCCIT cells and Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across the HOXA gene cluster.
    Polycomb Group Antibody Sampler Kit: Image 17 Expand Image
    Confocal immunofluorescent analysis of HeLa cells (left) and NTERA2 cells (right) using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
    Polycomb Group Antibody Sampler Kit: Image 18 Expand Image
    Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using Ezh2 (D2C9) XP® Rabbit mAb performed on the Leica BOND Rx.
    Polycomb Group Antibody Sampler Kit: Image 19 Expand Image
    CUT&Tag was performed with NCCIT cells and Ezh2 (D2C9) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across the HOXA gene cluster (upper), and the HOXD gene cluster (lower), known target genes of Ezh2 (see our ChIP-qPCR figure).
    Polycomb Group Antibody Sampler Kit: Image 20 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using RING1B (D22F2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Polycomb Group Antibody Sampler Kit: Image 21 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bmi1 (D20B7) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 22 Expand Image
    CUT&Tag was performed with NCCIT cells and Bmi1 (D20B7) XP® Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across the HOXA gene cluser (upper), and the HOXD gene cluster (lower), which are known target genes of Bmi1 (see our ChIP-qPCR figure).
    Polycomb Group Antibody Sampler Kit: Image 23 Expand Image
    CUT&RUN was performed with NCCIT cells and either Bmi1 (D20B7) XP® Rabbit mAb or CBX4 (E6L7X) Rabbit mAb #30559, using CUT&RUN Assay Kit #86652. DNA libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across HoxA gene (upper) and HoxD gene (lower), known target genes of CBX4 and Bmi1 (see additional figure containing CUT&RUN-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 24 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and Bmi1 (D20B7) XP® Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxA (upper) and HoxD (lower), known target genes of Bmi1 (see additional figure containing ChIP-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 25 Expand Image
    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb performed on the Leica® BOND™ Rx.
    Polycomb Group Antibody Sampler Kit: Image 26 Expand Image
    CUT&Tag was performed with NCCIT cells and Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across the HOXA gene cluster (upper), and HOXD gene cluster (lower).
    Polycomb Group Antibody Sampler Kit: Image 27 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using Ring1A (D2P4D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Polycomb Group Antibody Sampler Kit: Image 28 Expand Image
    Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SUZ12 (D39F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
    Polycomb Group Antibody Sampler Kit: Image 29 Expand Image
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 30 Expand Image
    Flow cytometric analysis of HeLa cells using RING1B (D22F2) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Polycomb Group Antibody Sampler Kit: Image 31 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lymphoma using Bmi1 (D20B7) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 32 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either Bmi1 (D20B7) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).
    Polycomb Group Antibody Sampler Kit: Image 33 Expand Image
    CUT&RUN was performed with NCCIT cells and treatment and either Bmi1 (D20B7) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Polycomb Group Antibody Sampler Kit: Image 34 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse lung using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 35 Expand Image
    Flow cytometric analysis of HeLa cells using SUZ12 (D39F6) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
    Polycomb Group Antibody Sampler Kit: Image 36 Expand Image
    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 37 Expand Image
    Confocal immunofluorescent analysis of COS-7 cells using Bmi1 (D20B7) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
    Polycomb Group Antibody Sampler Kit: Image 38 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse thymus using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 39 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP® Rabbit mAb or RING1B (D22F2) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. SUZ12 and RING1B are known to associate with each other on chromatin. The figure shows binding of both SUZ12 and RING1B across HOXD genes, which are known target genes of both SUZ12 and RING1B (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.
    Polycomb Group Antibody Sampler Kit: Image 40 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lymphoma using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 41 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either RING1B (D22F2) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795. RING1B and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both RING1B and H3K27me3 across HOXD genes, which are known target genes of RING1B (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product datasheet.
    Polycomb Group Antibody Sampler Kit: Image 42 Expand Image
    Flow cytometric analysis of K562 cells using Bmi1 (D20B7) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Polycomb Group Antibody Sampler Kit: Image 43 Expand Image
    Immunohistochemical analysis of paraffin-embedded human lymphoma using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb in the presence of non-methyl peptide (left) or K27 tri-methyl peptide (right).
    Polycomb Group Antibody Sampler Kit: Image 44 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
    Polycomb Group Antibody Sampler Kit: Image 45 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP® Rabbit mAb or RING1B (D22F2) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. SUZ12 and RING1B are known to associate with each other on chromatin. The figure shows binding of both SUZ12 and RING1B across HOXA genes (upper) and HOXD genes (lower), which are known target genes of SUZ12 (see additional figure containing ChIP-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 46 Expand Image
    Immunohistochemical analysis of paraffin-embedded 4T1 mouse syngeneic tumor using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 47 Expand Image
    Confocal immunofluorescent analysis of mouse hippocampus (left) and cerebellum (right) using Ezh2 (D2C9) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
    Polycomb Group Antibody Sampler Kit: Image 48 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either RING1B (D22F2) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. RING1B and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both RING1B and H3K27me3 across HOXA genes (upper) and HOXD genes (lower), which are known target genes of RING1B (see additional figure containing ChIP-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 49 Expand Image
    Flow cytometric analysis of Jurkat cells using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
    Polycomb Group Antibody Sampler Kit: Image 50 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SUZ12 (D39F6) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).
    Polycomb Group Antibody Sampler Kit: Image 51 Expand Image
    Confocal immunofluorescent analysis of HeLa cells using Ezh2 (D2C9) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (blue). Actin filaments were labeled with DY-554 phalloidin (red).
    Polycomb Group Antibody Sampler Kit: Image 52 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse brain using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 53 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either RING1B (D22F2) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).
    Polycomb Group Antibody Sampler Kit: Image 54 Expand Image
    CUT&RUN was performed with NCCIT cells and SUZ12 (D39F6) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxA1, a known target gene of SUZ12 (see additional figure containing CUT&RUN-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 55 Expand Image
    Flow cytometric analysis of human peripheral blood mononuclear cells untreated (left) and treated (right) with anti-human CD3 (10ug/ml, coated plates) and anti-human CD28 (5ug/ml) for 3 days at 37ºC using EZH2 (D2C9) XP® Rabbit mAb and co-stained with an anti-human CD3 antibody. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody.
    Polycomb Group Antibody Sampler Kit: Image 56 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse liver using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 57 Expand Image
    CUT&RUN was performed with NCCIT cells and either RING1B (D22F2) XP® Rabbit mAb or Bmi1 (D42B3) Rabbit mAb #5856, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. RING1B and Bmi1 are both PRC1 components. The figure shows binding across COMMD3 gene.
    Polycomb Group Antibody Sampler Kit: Image 58 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP® Rabbit mAb #5246 or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across MYT1, a known target gene of H3K27me3 (see additional figure containing ChIP-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 59 Expand Image
    CUT&RUN was performed with NCCIT cells and SUZ12 (D39F6) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxD (upper) and HoxA (lower), a known target gene of SUZ12 (see additional figure containing CUT&RUN-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 60 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across the MYT1 gene. For additional ChIP-seq tracks, please download the product datasheet.
    Polycomb Group Antibody Sampler Kit: Image 61 Expand Image
    Immunohistochemical analysis of paraffin-embedded mouse testis using Ezh2 (D2C9) XP® Rabbit mAb.
    Polycomb Group Antibody Sampler Kit: Image 62 Expand Image
    CUT&RUN was performed with NCCIT cells and either RING1B (D22F2) XP® Rabbit mAb or Bmi1 (D42B3) Rabbit mAb #5856, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. RING1B and Bmi1 are both PRC1 components. The figures show binding across HOXD (upper) and COMMD3 (lower) genes.
    Polycomb Group Antibody Sampler Kit: Image 63 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP® Rabbit mAb #5246 or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across chromosome 20 (upper), including MYT1 (lower), a known target gene of H3K27me3 (see additional figure containing ChIP-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 64 Expand Image
    CUT&RUN was performed with NCCIT cells and either SUZ12 (D39F6) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Polycomb Group Antibody Sampler Kit: Image 65 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from Hela cells and either Ezh2 (D2C9) XP® Rabbit mAb or Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. EZH2 and H3K27me3 are known to associate with each other on chromatin. The figure shows binding of both EZH2 and H3K27me3 across chromosome 20 (upper), including MYT1 gene (lower).
    Polycomb Group Antibody Sampler Kit: Image 66 Expand Image
    CUT&RUN was performed with NCCIT cells and either RING1B (D22F2) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Polycomb Group Antibody Sampler Kit: Image 67 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Polycomb Group Antibody Sampler Kit: Image 68 Expand Image
    Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either Ezh2 (D2C9) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Polycomb Group Antibody Sampler Kit: Image 69 Expand Image
    CUT&RUN was performed with HeLa cells and Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figure shows binding across TCEA2 gene.
    Polycomb Group Antibody Sampler Kit: Image 70 Expand Image
    CUT&RUN was performed with NCCIT cells and Ezh2 (D2C9) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across HoxA1, a known target gene of Ezh2 (see additional figure containing CUT&RUN-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 71 Expand Image
    CUT&RUN was performed with HeLa cells and Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 20 (upper), including TCEA2 gene (lower).
    Polycomb Group Antibody Sampler Kit: Image 72 Expand Image
    CUT&RUN was performed with NCCIT cells and Ezh2 (D2C9) XP® Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 7 (upper), including HoxA genes (lower), a known cluster of EZH2 target genes (see additional figure containing CUT&RUN-qPCR data).
    Polycomb Group Antibody Sampler Kit: Image 73 Expand Image
    CUT&RUN was performed with HeLa cells and either Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human MYT-1 Exon 1 Primers, SimpleChIP® Human MyoD1 Exon 1 Primers, and SimpleChIP® Human GAPDH Exon 1 Primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
    Polycomb Group Antibody Sampler Kit: Image 74 Expand Image
    CUT&RUN was performed with NCCIT cells and either Ezh2 (D2C9) XP® Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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