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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SON (F3Y3D) Rabbit mAb #43422

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 300
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SON (F3Y3D) Rabbit mAb recognizes endogenous levels of total SON protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human SON protein.

    Background

    Nuclear speckles (NS) are distinct biomolecular condensates consisting of nuclear proteins and RNA molecules. Although their exact biological function is not fully understood, NS are believed to function as hubs for mRNA splicing and potentially transcription (1). The splicing factors serine/arginine repetitive matrix protein 2 (SRRM2) and SON are necessary for NS formation (2). SRRM2 and SON undergo phase separation and function as scaffolding proteins, recruiting additional splicing factors and promoting NS assembly (3). SON has been shown to be critical for maintaining pluripotency in human embryonic stem cells (hESCs) by splicing key regulatory gene transcripts (4). SON-mediated RNA splicing has also been shown to be essential for the survival of glioblastoma multiforme (GBM) cells, suggesting its use as a therapeutic target (5). 
    For Research Use Only. Not for Use in Diagnostic Procedures.
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