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56511
Tau Mouse Model Neuronal Viability IF Sampler Kit
一次抗体
Antibody Samplerキット

Tau Mouse Model Neuronal Viability IF Sampler Kit #56511

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Confocal immunofluorescent analysis of mouse medulla oblangata using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (green). Blue = DAPI #4083 (fluorescent DNA dye).

Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.

Immunohistochemical analysis of paraffin-embedded Alzheimer's brain, using Tau (Tau46) Mouse mAb.

Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Tau (Tau46) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.

Confocal immunofluorescent analysis of mouse hippocampus (left), cortex (middle), and cerebellum (right) using NeuN (D4G4O) XP® Rabbit mAb (green). アクチンフィラメントをDyLight™ 554 Phalloidin #13054 (赤) で染色しています。DRAQ5® #4084を用いてDNAを染色し、青の疑似カラーで示しました。

Confocal immunofluorescent analysis of mouse brain (left) and mouse retina (right) using Synaptophysin (7H12) Mouse mAb (IF Formulated) (green) and CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #5715 (red, left). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of rat cerebellum and retina using PSD95 (D27E11) XP® Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). DRAQ5® #4084を用いてDNAを染色し、青の疑似カラーで示しました。

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3 (Asp175) Antibody compared to a nonspecific negative control antibody (red).

Flow cytometric analysis of serum-starved Neuro-2a cells, untreated (blue) or treated with Staurosporine #9953 (1 μM, 3 hr; green), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412を二次抗体として使用しました。

Confocal immunofluorescent analysis of mouse Tg2576 brain, which overexpresses mutant human APP695. Sections were labeled with GFAP (E6N9L) Mouse mAb (green, mouse IgG2a), β-Amyloid (D3D2N) Mouse mAb #15126 (red, mouse IgG1), and HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) #3892 (magenta, rabbit IgG). サンプルは、ProLong® Gold Antifade Reagent with DAPI #8961 (青) で封入しています。

Flow cytometric analysis of NIH/3T3 cells (red) and 32D cells (blue), using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific).

Confocal immunofluorescent analysis of dentate gyrus in wild-type mouse brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (green). Antibody was pre-incubated with a non-phospho-Tau peptide (left), a phospho-Tau (Thr205) peptide (center), or without peptide (right) to confirm phospho-specificity. サンプルは、ProLong® Gold Antifade Reagent with DAPI #8961 (青) で封入しています。

Western blot analysis of extracts from mouse brain, untreated (-) or phosphatase-treated (+), and rat brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb (upper) and Tau (D1M9X) Rabbit mAb #46687 (lower).

Western blot analysis of extracts from mouse and rat brain, using Tau (Tau46) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using NeuN (D4G4O) XP® Rabbit mAb.

Western blot analysis of extracts from human cerebellum and rat brain using PSD95 (D27E11) XP® Rabbit mAb.

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right), labeled with Cleaved Caspase-3 (Asp175) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin #8953 (red). 青の疑似カラー=DRAQ5®​ (DNA蛍光染色試薬)。

Flow cytometric analysis of serum-starved H-4-II-E cells, untreated (blue) or treated with Staurosporine #9953 (1 μM, 3 hr; green), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412を二次抗体として使用しました。

Immunoprecipitation of GFAP protein from mouse brain tissue extracts. Lane 1 is 10% input, lane 2 is Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656, and lane 3 is GFAP (E6N9L) Mouse mAb. Western blot analysis was performed GFAP (E6N9L) Mouse mAb.

Tg2576マウス (変異型ヒトAPP695を過剰発現するアルツハイマー病モデルマウス) の脳組織切片を、Sections were first labeled with HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (green) and APP/β-Amyloid (NAB228) Mouse mAb #2450 (yellow). 空いている二次結合部位をMouse (G3A1) mAb IgG1 Isotype Control #5415で遮断した後、切片をGFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (赤) でインキュベートしました。核はHoechst 33342 #4082 (青) で標識しました。

Immunohistochemical analysis of paraffin-embedded human Alzheimer's disease brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb in the presence of non-phospho-Tau (Thr205) peptide (left) or phospho-Tau (Thr205) peptide (right).

Immunohistochemical analysis of paraffin-embedded mouse colon (myenteric plexus) using NeuN (D4G4O) XP® Rabbit mAb.

Confocal immunofluorescent analysis of Neuro-2a cells, untreated (left, negative) or treated with Staurosporine #9953 (1 μM, 3 hr; right, positive), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) (green). アクチンフィラメントをDyLight™ 554 Phalloidin #13054 (赤) で染色しています。DRAQ5® #4084を用いてDNAを染色し、青の疑似カラーで示しました。

Western blot analysis of extracts from various tissues and cell lines using GFAP (E6N9L) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Confocal immunofluorescent analysis of 32D cells (left) and C2C12 cells (right), using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (green). DRAQ5® #4084を用いてDNAを染色し、青の疑似カラーで示しました。

Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human glioblastoma multiform using NeuN (D4G4O) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic and perinuclear localization in apoptotic cells (low and high magnification), using Cleaved Caspase-3 (Asp175) Antibody.

Immunohistochemical analysis of paraffin-embedded 3T3 cell pellet, untreated (left, negative) or treated with Staurosporine #9953 (right, positive), using Cleaved PARP (Asp214)(D6X6X) mAb (Rodent Specific).

Western blot analysis of extracts from mouse brain using GFAP (E6N9L) Mouse mAb as the primary antibody. Various anti-mouse isotype specific antibodies (upper) and Anti-mouse IgG, HRP-linked Antibody #7076 (lower) were used as secondary antibodies.

Immunohistochemical analysis of paraffin-embedded mouse spleen using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific).

Immunohistochemical analysis of paraffin-embedded mouse small intestine using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.

Western blot analysis of extracts from mouse brain, C2C12 cells, and rat brain using NeuN (D4G4O) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis using Cleaved caspase-3 (Asp175) antibody on SignalSlide™ Cleaved Caspase-3 IHC controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).

Immunohistochemical analysis of paraffin-embedded E14 rat embryo using Cleaved PARP (Asp214)(D6X6X) mAb (Rodent Specific).

Immunohistochemical analysis of paraffin-embedded LL2 syngeneic tumor using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific).

Immunoprecipitation of Phospho-Tau (Thr205) from mouse brain lysates. Lane 1 mouse brain lysate immunoprecipitation, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, lanes 3, 4, and 5 represent three dilutions of the immunoprecipitation of mouse brain lysates at 1:50, 1:100, 1:200 respectively, and lane 6 is mouse brain lysate. Western blot analysis was performed using Phospho-Tau (Thr205) (E7D3E) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).

Immunohistochemical analysis of paraffin-embedded H-4-II-E cell pellet, untreated (left, negative) or treated with Staurosporine #9953 (right, positive), using Cleaved PARP (Asp214)(D6X6X) mAb (Rodent Specific).

Western blot analysis of cell extracts from Baf3, 32D, and mouse spleen using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific).

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (3hrs, 1 µM in vivo) or cytochrome c-treated (1hr, 0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).

Immunohistochemical analysis of paraffin-embedded mouse ovary using Cleaved PARP (Asp214)(D6X6X) mAb (Rodent Specific).

Immunohistochemical analysis of paraffin-embedded mouse spleen using Cleaved PARP (Asp214)(D6X6X) mAb (Rodent Specific).

Immunoprecipitation of Cleaved PARP (Asp214) from Neuro-2a cell extracts treated with Staurosporine #9953 (1 μM, 3 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific). Western blot was perform using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific). Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

Western blot analysis of extracts from serum-starved Neuro-2a cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from serum-starved H-4-II-E cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 6 hr; +), using Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 56511T
製品番号 サイズ 価格 在庫
56511T
1 Kit  (9 x 20 µl)

Product Includes Quantity アプリケーション 種交差性 MW(kDa) Isotype
Phospho-Tau (Thr205) (E7D3E) Rabbit mAb 49561 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R 50-80 Rabbit IgG
Tau (Tau46) Mouse mAb 4019 20 µl
  • WB
  • IHC
H M R 50 to 80 Mouse IgG1
NeuN (D4G4O) XP® Rabbit mAb 24307 20 µl
  • WB
  • IHC
  • IF
H M R 46-55 Rabbit IgG
Synaptophysin (7H12) Mouse mAb (IF Formulated) 9020 20 µl
  • IF
H M R Mouse IgG1
PSD95 (D27E11) XP® Rabbit mAb 3450 20 µl
  • WB
  • IF
H M R 95 Rabbit IgG
Cleaved Caspase-3 (Asp175) Antibody 9661 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Mk 17, 19 ラビット 
Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) 94885 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
M R 89 Rabbit IgG
GFAP (E6N9L) Mouse mAb 34001 20 µl
  • WB
  • IP
  • IF
H M R 50 Mouse IgG2a
HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) 3892 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
M R 80 Rabbit IgG

Product Description

The Tau Mouse Model Neuronal Viability IF Sampler Kit provides an economical means of detecting proteins to confirm neuronal viability and surrounding astrocytes and microglia in mouse models by immunofluorescence.

特異性 / 感度

Each antibody in the Tau Mouse Model Neuronal Viability IF Sampler Kit detects endogenous levels of its target protein. Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein and also cross-reacts with MAP2 at 280 kDa. Tau (Tau46) Mouse mAb is predicted to detect all six isoforms of tau based on the amino acid sequence. Phospho-Tau (Thr205) (E7D3E) Rabbit mAb recognizes endogenous levels of tau protein only when phosphorylated at Thr205. Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (Rodent Specific) recognizes endogenous levels of the large fragment (89 kDa) of rodent PARP protein only when cleaved at Asp214. HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) does not recognize human HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels. Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases. This antibody detects non-specific caspase substrates by western blot. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Nuclear background may be observed in rat and monkey samples.

使用抗原 / 精製方法

Monoclonal and polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln53 of human PSD95, Asp214 of rodent PARP1, Leu310 of mouse HS1, amino-terminal residues adjacent to Asp175 of human caspase-3, a phosphopeptide surrounding Thr205 of human tau, recombinant protein specific to the carboxy terminus of human SYP and the amino terminus of human NeuN, native GFAP purified from pig spinal cord, and native bovine tau and the epitope maps to the carboxy-terminus of the protein.

バックグラウンド

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Neurofibrillary tangles are a major pathological hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau, including phosphorylation of tau at Thr205 (1,2). Research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (4). Glial fibrillary acidic protein (GFAP) is the main intermediate filament in mature brain astroglial and radial glial cells. GFAP plays an important role in modulating astroglial motility and shape (5). HS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (6). Previous work identifying markers of specific brain cell types using RNA-seq has shown HS1 to be a useful and specific tool to study microglia (7). Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that occurs in presynaptic vesicles of neurons (8). Postsynaptic density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (9,10). Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, including nuclear enzyme poly (ADP-ribose) polymerase (PARP) (11). PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (12). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (13).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Wang, J. et al. (2000) Zhongguo Yi Xue Ke Xue Yuan Xue Bao 22, 120-3.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Mullen, R.J. et al. (1992) Development 116, 201-11.
  5. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  6. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
  7. Zhang, Y. et al. (2014) J Neurosci 34, 11929-47.
  8. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci U S A 83, 3500-4.
  9. Cao, J. et al. (2005) J. Cell Biol 168, 117-26.
  10. Chetkovich, D.M. et al. (2002) J. Neurosci. 22, 6415-25.
  11. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  12. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.
  13. Oliver, F.J. et al. (1998) J Biol Chem 273, 33533-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

研究用のみ。診断手順では使用しません。

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XPはCell Signaling Technology, Inc.の商標です。

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