Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-Bond, IHC-P

REACTIVITY:

H M Mk

SENSITIVITY:

Endogenous

MW (kDa):

55

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q9GZV5

Entrez-Gene Id:

25937

Product Information

Product Usage Information

This product is the carrier free version of product #72804. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #72804

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total TAZ protein. This antibody does not cross-react with YAP protein. Species cross-reactivity for IHC-P is human only.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinantly expressed full-length human TAZ protein.

Background

TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ is negatively regulated via phosphorylation by LATS1/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development (4).

  1. Kanai, F. et al. (2000) EMBO J 19, 6778-91.
  2. Hong, J.H. et al. (2005) Science 309, 1074-8.
  3. Hong, J.H. and Yaffe, M.B. (2006) Cell Cycle 5, 176-9.
  4. Lei, Q.Y. et al. (2008) Mol Cell Biol 28, 2426-36.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 1
#49756

TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free)

Western Blotting Image 1: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from various cell lines using TAZ (E9J5A) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using TAZ (E9J5A) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using TAZ (E9J5A) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma (left), colon carcinoma case 1 (middle), or colon carcinoma case 2 (right), using TAZ (E9J5A) XP® Rabbit mAb (top) or YAP (D8H1X) XP® Rabbit mAb #14074 (bottom). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded BEN cell pellet, untransfected (left, YAP/TAZ low-expressing), YAP-transfected (middle), or TAZ-transfected (right), using TAZ (E9J5A) XP® Rabbit mAb (top) or YAP (D8H1X) XP® Rabbit mAb #14074 (bottom). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using TAZ (E9J5A) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TAZ (E9J5A) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using TAZ (E9J5A) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 cell pellet (left, positive) or RL cell pellet (right, negative) using TAZ (E9J5A) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: TAZ (E9J5A) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TAZ (E9J5A) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.