Revision 7
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, IHC-Bond, IHC-P

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

40-45

Source/Isotype:

Rabbit IgG

UniProt ID:

#B7Z8K6

Entrez-Gene Id:

28526

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
IHC Leica Bond 1:100 - 1:400
Immunohistochemistry (Paraffin) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #61129.

Specificity / Sensitivity

TRDC/TCRδ (E2E9T) XP® Rabbit mAb recognizes endogenous levels of total TRDC/TCRδ protein. This antibody does not cross-react with human TRAC/TCRα constant, TRBC1/TCRβ constant region 1, TRBC2/TCRβ constant region 2, or TRGC1/TCRγ constant region 1.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human TRDC/TCRδ recombinant protein.

Background

The γδ T Cell Receptor (TCR) is a heterodimer composed of a gamma chain and a delta chain expressed on the surface of T cells which participates in antigen recognition (1,2). Binding of gamma-delta TCR to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3 chains of the antigen (3). Unlike αβ T cells, human γδ T cells recognize and kill transformed cells independently of human leukocyte antigen (HLA) restriction (3,4). γδ T lymphocytes develop largely in the thymus, generating their defining receptor via RAG-mediated rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes (3). In addition to the T cell receptor, γδ T cells usually also express activating natural killer (NK) receptors, which bind to stress-inducible surface molecules frequently expressed on malignant cells (4).

  1. Brenner, M.B. et al. Nature 322, 145-9.
  2. Davis, M.M. and Bjorkman, P.J. (1988) Nature 334, 395-402.
  3. Vantourout, P. and Hayday, A. (2013) Nat Rev Immunol 13, 88-100.
  4. Kabelitz, D. et al. (2020) Cell Mol Immunol 17, 925-939.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 7
#55750

TRDC/TCRδ (E2E9T) XP® Rabbit mAb

Western Blotting Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human TRAC/TCRα constant protein (hTRAC/TCRα constant-Myc/DDK; +), Myc/DDK-tagged full-length human TRBC1/TCRβ constant region 1 protein (hTRBC1/TCRβ constant region 1-Myc/DDK; +), Myc/DDK-tagged full-length human TRBC2/TCRβ constant region 2 protein (hTRBC2/TCRβ constant region 2-Myc/DDK; +), Myc/DDK-tagged full-length human TRDC/TCRδ constant protein (hTRDC/TCRδ constant-Myc/DDK; +), and Myc/DDK-tagged full-length human TRGC1/TCRγ constant region 1 protein (hTRGC1/TCRγ constant region 1-Myc/DDK; +), using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Western blot analysis of extracts from PEER and Jurkat cells using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunoprecipitation of TRDC/TCRδ protein from PEER cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Western blot analysis was performed using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
Immunohistochemistry Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb performed on the Leica® BOND Rx.
Immunohistochemistry Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human spleen using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 2: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human thymus using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 3: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human tonsil using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 4: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 5: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 6: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 7: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.
Immunohistochemistry Image 8: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Immunohistochemistry Image 9: TRDC/TCRδ (E2E9T) XP® Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded PEER cell pellet (left, positive) or Raji cell pellet (right, negative) using TRDC/TCRδ (E2E9T) XP® Rabbit mAb.