The Tyro/Axl/Mer Activation Sampler Kit provides an economical means of detecting the activation of TAM family members using phospho-specific and control antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Specificity / Sensitivity
Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit mAb detects immunoprecipitated or transfected levels of Axl/Mer/Tyro3 protein when phosphorylated at Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681). This antibody may cross-react with other phosphorylated tyrosine proteins. Phospho-Axl (Tyr702) (D12B2) Rabbit mAb detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases. Axl (C89E7) Rabbit mAb, Mer (D21F11) XP® Rabbit mAb, Tyro3 (D38C6) Rabbit mAb, and GAS6 (D3A3G) Rabbit mAb detected endogenous levels of each of the corresponding target proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to phospho-Tyr698/Tyr749 of human Axl/Mer, phospho-Tyr702 of human Axl, a recombinant fragment of human Axl, synthetic peptides corresponding to His925 of human Mer, Thr874 of human Tyro3, and recombinant protein specific to the amino terminus of human GAS6.
Axl, Mer and Tyro3 are three members of the TAM family receptor tyrosine kinase that share a common NCAM (neural adhesion molecule)-related extracellular domain and a conserved intracellular tyrosine kinase domain. These receptors bind common homologous vitamin K dependent protein GAS6 and protein S to activate downstream signaling pathways (1). TAM family receptors are involved in the development of immune, nervous, vascular and reproductive systems, autoimmune disease, cancer drug resistance and tumor immunity response (2-5). Axl (Tyr698), Axl (Tyr702), Mer Tyr(749) and Tyro3 (Tyr681) are conserved autophosphorylation sites located in the activation loop of the respective tyrosine kinase domains. Phosphorylation at these sites is required for full kinase activation of each of the corresponding receptors (6,7).