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56548
VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free) #56548

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines and tissues using VISTA (D5L5T) XP® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse CT26 syngeneic tumor using VISTA (D5L5T) XP® Rabbit mAb performed on the Leica® Bond Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse lung using VISTA (D5L5T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse spleen using VISTA (D5L5T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse colon using VISTA (D5L5T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded EL4 cell pellet (left, positive) or 3T3 cell pellet (right, negative) using VISTA (D5L5T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse colon using VISTA (D5L5T) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DRAQ5® #4084 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) and HeLa cells (right, negative) using VISTA (D5L5T) XP® Rabbit mAb (green), β-Actin Mouse mAb (red), and DRAQ5® #4084 (blue). Data were generated using the standard formulation of this product.
To Purchase # 56548
製品# サイズ 数量 価格 在庫
56548SF
100 µg

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 45-65
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #54979. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #54979.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

VISTA (D5L5T) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total VISTA protein. This antibody is not recommended for immunohistochemical analysis of human tissues. Instead, VISTA (D1L2G™) XP® Rabbit mAb #64953 is suggested.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val291 of mouse VISTA protein.

Background

VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7).
  1. Wang, L. et al. (2011) J Exp Med 208, 577-92.
  2. Flies, D.B. et al. (2011) J Immunol 187, 1537-41.
  3. Lines, J.L. et al. (2014) Cancer Res 74, 1924-32.
  4. Wang, L. et al. (2014) Proc Natl Acad Sci U S A 111, 14846-51.
  5. Liu, J. et al. (2015) Proc Natl Acad Sci U S A 112, 6682-7.
  6. Le Mercier, I. et al. (2014) Cancer Res 74, 1933-44.
  7. Lines, J.L. et al. (2014) Cancer Immunol Res 2, 510-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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