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10483
PTMScan®N-Terminal Acetyl Motif [N-Ac] Kit
Proteomic Analysis Products

PTMScan®N-Terminal Acetyl Motif [N-Ac] Kit #10483

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The Motif Logo was generated from an N-Terminal AcetylScan® LC-MS/MS experiment using 839 nonredundant tryptic peptides derived from mouse liver, brain, and embryo tissues immunoprecipitated with PTMScan® N-Terminal Acetyl Motif Immunoaffinity Beads. The logo represents the relative frequency of amino acids in each position starting from the N-terminal residue of each peptide within this data set.

Product Includes Cap Color Volume (with Count)
N-Terminal Acetyl Motif [N-Ac] Immunoaffinity Beads 10 x 80 µl
PTMScan® IAP Buffer (10X) 9993 10 x 600 µl
PTMScan® Limited Use License 1 x 1 ea

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method is included with the kit.

Storage:

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/services/index.html

Background

Various forms of N-terminal protein processing exist in eukaryotes that target the starting methionine amino acid. One such modification is acetylation of the N-terminal methionine accepted from acetyl-CoA. This is mediated by N-terminal acetyltransferases (Nats) and has been found to be present on a wide variety of proteins estimated from 50 to more than 80% of proteins in some cases. There are six identified Nats that are well conserved from yeast to humans (1). This widely observed modification has been implicated in a variety of protein processes from translation regulation, stability, folding, localization, transcriptional control, and protein protein interactions. It also plays a role in Histone tail modifications (2,3,4,5).

  1. Liu, C.C. et al. (2013) PLoS One 8, e58681.
  2. Kalvik, T.V. and Arnesen, T. (2013) Oncogene 32, 269-76.
  3. Arnesen, T. et al. (2009) Proc Natl Acad Sci U S A 106, 8157-62.
  4. Van Damme, P. et al. (2012) Proc Natl Acad Sci U S A 109, 12449-54.
  5. Ree, R. et al. (2018) Exp Mol Med 50, 90.
For Research Use Only. Not For Use In Diagnostic Procedures.

AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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