ただ今実施中のプロモーション | 詳細はこちら >>
12170
PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit
Proteomic Analysis Products
PTMScan

PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit #12170

Reviews ()
Citations (0)
PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit: Image 1

This chart shows the underlying CK2 substrate-like motifs in a PhosphoScan® LC-MS/MS experiment of tryptic peptides generated from Jurkat cells treated with Calyculin A #9902 and pervanadate, and immunoprecipitated using PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads. Of the 354 motif-containing peptides, 54% contained phospho-serine and 46% contained phospho-threonine. The proportion of peptides with aspartate or glutamate at the +1 position is 83% and at the +3 position is 73%. Peptides with aspartate or glutamate at both the +1 and +3 positions is 63%.

PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit: Image 2

The Motif Logo was generated from a PhosphoScan® LC-MS/MS experiment using 471 nonredundant tryptic peptides derived from Jurkat cells treated with Calyculin A #9902 and pervanadate, and immunoprecipitated using PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads. This PhosphoSitePlus® logo represents the enrichment of an amino acid at a residue position relative to the abundance of that amino acid in the background, in this case, the human proteome. Residues represented at the top of the logo are the most enriched, whereas those below the y-axis occur less frequently in the sample than would be expected in the proteome. The PSP logo reflects the specificity of the antibody for the S*/T*DXE motif. For more information on motif analysis using PSP, please visit www.phosphosite.org.

Product Includes Volume (with Count)
PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Immunoaffinity Beads 10 x 80 µl
PTMScan® IAP Buffer (10X) 9993 10 x 600 µl
PTMScan® Limited Use License 1 x 1 ea

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method are included with the kit.

Storage

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/common/content/content.jsp?id=ptmscan-services.

Background

PhosphoScan® Technology employs a phospho-residue (Tyr, Ser, Thr) motif antibody for phospho-peptide immunoaffinity purification from cell extracts combined with LC tandem MS/MS to identify and quantify changes in phosphorylation levels (1). Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric serine/threonine protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer and other diseases (2-6). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated serine or threonine residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position, followed by the residue at the +1 position (7).

  1. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  2. Pinna, L.A. and Allende, J.E. (2009) Cell Mol Life Sci 66, 1795-9.
  3. St-Denis, N.A. and Litchfield, D.W. (2009) Cell Mol Life Sci 66, 1817-29.
  4. Trembley, J.H. et al. (2009) Cell Mol Life Sci 66, 1858-67.
  5. Perez, D.I. et al. (2011) Med Res Rev 31, 924-54.
  6. Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
  7. Meggio, F. and Pinna, L.A. (2003) FASEB J 17, 349-68.

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not For Use In Diagnostic Procedures.
AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected] For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]
Powered by Translations.com GlobalLink OneLink SoftwarePowered By OneLink