Revision 5

#43079Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, IHC-Bond, IHC-P, FC-FP

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

35

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q16633

Entrez-Gene Id:

5450

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
IHC Leica Bond 1:200 - 1:800
Immunohistochemistry (Paraffin) 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized) 1:1600 - 1:6400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #65452.

Specificity / Sensitivity

BOB-1/OBF-1 (E5K1D) Rabbit mAb recognizes endogenous levels of total BOB-1/OBF-1 protein. Non-specific staining was observed in pancreatic islets by immunohistochemistry.

Species Reactivity:

Human

Species predicted to react based on 100% sequence homology

Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro209 of human BOB-1/OBF-1 protein.

Background

B cell Oct binding factor-1 (BOB-1/OBF-1) is a B cell restricted transcriptional coactivator. BOB-1 facilitates transactivation of immunoglobulins and other B cell specific genes through the binding and activation of the transcription factors Oct-1 and Oct-2 (1-4). Research studies have demonstrated that BOB-1 expression is required for antigen-dependent B cell maturation (5-7). In pathological conditions such as classical Hodgkin disease, loss of BOB-1 expression is thought, in part, to contribute to the defect in immunoglobulin gene expression by Hodgkin and Reed Sternberg cells (8,9). In the context of multiple myeloma, overexpression of BOB-1 has been shown to contribute to malignant plasma cell growth, in part, through enhanced transactivation of TNFRSF17/BCMA (10).

  1. Strubin, M. et al. (1995) Cell 80, 497-506.
  2. Laumen, H. et al. (2000) Eur J Immunol 30, 458-69.
  3. Gstaiger, M. et al. (1995) Nature 373, 360-2.
  4. Brunner, C. and Wirth, T. (2006) Nucleic Acids Res 34, 1807-15.
  5. Hess, J. et al. (2001) Mol Cell Biol 21, 1531-9.
  6. Kim, U. et al. (1996) Nature 383, 542-7.
  7. Schubart, D.B. et al. (1996) Nature 383, 538-42.
  8. Re, D. et al. (2001) Cancer Res 61, 2080-4.
  9. Stein, H. et al. (2001) Blood 97, 496-501.
  10. Zhao, C. et al. (2008) Oncogene 27, 63-75.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 5
#43079

BOB-1/OBF-1 (E5K1D) Rabbit mAb

Western Blotting Image 1: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Western blot analysis of extracts from various cell lines using BOB-1/OBF-1 (E5K1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, BOB-1/OBF-1 protein is not detected in either HDLM-2 or HeLa cells.
Western Blotting Image 2: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human BOB-1 protein (hBOB-1-Myc/DDK; +), using BOB-1/OBF-1 (E5K1D) Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation Image 1: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunoprecipitation of BOB-1/OBF-1 protein from Daudi cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BOB-1/OBF-1 (E5K1D) Rabbit mAb. Western blot analysis was performed using BOB-1/OBF-1 (E5K1D) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.
Immunohistochemistry Image 1: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb performed on the Leica BOND RX.
Immunohistochemistry Image 2: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb performed on the Leica BOND RX.
Immunohistochemistry Image 3: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human bronchioloalveolar adenocarcinoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb performed on the Leica BOND RX.
Immunohistochemistry Image 1: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Immunohistochemistry Image 2: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Immunohistochemistry Image 3: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Immunohistochemistry Image 4: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human thymus using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Immunohistochemistry Image 5: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human uterus using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Immunohistochemistry Image 6: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human lymph node using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Immunohistochemistry Image 7: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using BOB-1/OBF-1 (E5K1D) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 8: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Immunohistochemical analysis of paraffin-embedded Daudi cell pellet (left, positive) or HeLa cell pellet (right, negative) using BOB-1/OBF-1 (E5K1D) Rabbit mAb.
Flow Cytometry Image 1: BOB-1/OBF-1 (E5K1D) Rabbit mAb Expand Image
Flow cytometric analysis of HeLa cells (blue, negative) and Daudi cells (green, positive) using BOB-1/OBF-1 (E5K1D) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.