Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IHC-P, IF-F, IF-IC

REACTIVITY:

H M

SENSITIVITY:

Endogenous

MW (kDa):

270

Source/Isotype:

Rabbit IgG

UniProt ID:

#P46821

Entrez-Gene Id:

4131

Product Information

Product Usage Information

Application Dilution
Immunohistochemistry (Paraffin) 1:1500 - 1:6000
Immunofluorescence (Frozen) 1:200 - 1:400
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

MAP1B (E8S8R) Rabbit mAb (IF Formulated) recognizes endogenous levels of total MAP1B protein.

Species Reactivity:

Human, Mouse

Species predicted to react based on 100% sequence homology

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1570 of human MAP1B protein.

Background

Microtubule-associated protein 1B (MAP1B) is a member of the high molecular weight microtubule binding phosphoprotein family, along with MAP1A (1). MAP1B is highly expressed in neurons, predominantly during development, where it regulates axonal guidance and elongation through its influence on microtubule dynamics (1,2). In mature neurons, MAP1B plays a role in regulation and structure of dendritic spines in glutamatergic synapses. MAP1B deficient neurons have been shown to display structural presynaptic deficiencies, including a decrease in density at synaptic terminals and disruption of synaptic vesicle fusion events (2). Mutations in MAP1B have been linked to developmental disorders affecting cognitive ability through a deficit in white matter and compromised axons (3).

  1. Gordon-Weeks, P.R. and Fischer, I. (2000) Microsc Res Tech 48, 63-74.
  2. Bodaleo, F.J. et al. (2016) Sci Rep 6, 30069.
  3. Walters, G.B. et al. (2018) Nat Commun 9, 3456.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

使用に関する制限

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Revision 1
#80531

MAP1B (E8S8R) Rabbit mAb (IF Formulated)

Immunohistochemistry Image 1: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 2: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 3: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 4: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 5: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 6: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the skin using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 7: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using MAP1B (E8S8R) Rabbit mAb (IF Formulated).

Immunohistochemistry Image 8: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 9: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 10: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image

Immunohistochemical analysis of paraffin-embedded normal human brain using MAP1B (E8S8R) Rabbit mAb (IF Formulated).

Immunohistochemistry Image 11: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human kidney using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 12: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human pancreas using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 13: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human placenta using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 14: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded human appendix using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 15: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human testis using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 16: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human small intestine using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 17: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human prostate using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 18: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 19: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse testis using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 20: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse spleen using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 21: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse ovary using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 22: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse liver using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunohistochemistry Image 23: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Immunohistochemical analysis of paraffin-embedded A549 cell pellet (left, positive) or NB-4 cell pellet (right, negative) using MAP1B (E8S8R) Rabbit mAb (IF Formulated).
Immunofluorescence Image 1: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse retina labeled with MAP1B (E8S8R) Rabbit mAb (IF Formulated) (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, yellow), GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 2: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum labeled with MAP1B (E8S8R) Rabbit mAb (IF Formulated) (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, yellow), GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 1: MAP1B (E8S8R) Rabbit mAb (IF Formulated) Expand Image
Confocal immunofluorescent analysis of A549 cells (left, positive) and OVCAR-5 (NIH) cells (right, negative) using MAP1B (E8S8R) Rabbit mAb (IF Formulated) (green), DyLight 650 Phalloidin #12956 (red), and DAPI #4083 (blue).