Technical support
Results (662)
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
SignalStar Multiplex IHCのキットと試薬は、抗体と蛍光色素の組み合わせや抗体の染色順について最適化されています。As tissues vary in quality and expression level of biomarkers, increasin...
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. 次にその例を挙げます。
- β-Actin (13E5) Rabbit mAb #4970
- β-Actin (D6A8) Rabbit mAb #...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
コンカナバリンAビーズが、CUT&RUN実験で「凝集する」 ことがあるのですが、注意事項はありますか？
Concanavalin Aビーズは、特に細胞が溶解された時に凝集しやすくなります。To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
CSTのCUT&RUN Assay Kitは単離した核でも使用できますか？
CSTのCUT&RUN Assay Kitは全細胞で使用できるように最適化されており、接着細胞、浮遊細胞を問わず多くの細胞株で使用できます。While it is not typically necessary to pre-isolate cell nucle...
What isoforms does the ZBP1 Antibody #60968 recognize?
ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorigenesis and innate imm...
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...
What controls should I include when performing a Simple Western™ experiment?
In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.
1. A no lysate control. This includes the primary antibody at t...
How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?
To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After digestion, you should ...
Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?
Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammator...
Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?
While the SignalStain® DAB Substrate Kit #8059 does have a recommended storage temperature of 4°C, we have found tha...
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
- Phospho-Akt (Ser473) (D9E) XP® Rabb...
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samples. These are p44/4...
CUT&RUNで得られるDNAの予測されるサイズ分布を教えてください。
When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosomal ladder of DNA frag...
Which Cas9 antibody is the most sensitive by western immunoblot?
The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejuni. The Cas9 (7A9...
Why is there a difference in the amino acid region of Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 and Human ACE2 (18-615) Recombinant Protein (8xHis-Tag) #73775?
The slight difference in the amino acid span of these products is not significant for binding to SARS-CoV-2. The Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 covers the entire...
What is the benefit of carrier vs. carrier free cytokines?
Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...
Why am I seeing a 32 kDa band with the β-Actin Antibody #4967?
β-Actin can be cleaved by caspase-3 between Asp244 and Gly245, which will result in a 32kDa fragment and a 15kDa fragment. Due to the location of its antigen, our β-Actin Antibody #4967 will detect bo...
Which ribosomal protein S6 (rpS6) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for ribosomal protein S6 (rpS6) that are validated for IHC-P with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse...
Can the Senescence β-Galactosidase Staining Kit #9860 be used with frozen tissue?
While we have not tested the Senescence β-Galactosidase Staining Kit #9860 with frozen tissue in-house, we have spoken with numerous customers who have done so with success using our recommended cell ...
Can I quantify my samples using the Senescence β-Galactosidase Staining Kit #9860?
The Senescence β-Galactosidase Staining Kit #9860 is intended to be a qualitative assay to determine if cells are senescent. We have not determined a means of quantification in-house. However, we usua...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?
We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 alone and when phospho...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
Does the amino acid sequence of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (319-591) being longer than the amino acid sequence of other RBD products (318-541) affect its binding activity with ACE2?
The additional amino acids found in SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 should not affect the binding of RBD to ACE2 because the RBD region ...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. この過剰な染色が正しいどうかを、どのように確認すれば良いですか？
最適化の過程で、蛍光染色では発色染色よりも高い陽性率 (%) を示す可能性があることがわかりました。To ensure any excess staining is specific, confirm that the correct sub...
Does Cell Lysis Buffer (10X) #9803 work with the Bradford assay?
Cell Lysis Buffer (10X) #9803 will interfere with the Bradford protein assay. This is because it contains detergent. To make the assay somewhat compatible, dilute your protein quantitation samples at ...
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
1回目のイメージングラウンドでは、染色完了後、最大8時間後までは強いシグナルを示すと考えられます。For Imaging Round 2, imaging should be performed as close to the completion of staini...
Why should I use the Protein A (HRP Conjugate) #12291 in place of the secondary antibody in my western blot for IP experiments?
Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy and light chain.