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CSTビデオライブラリから、皆様の実験手技の改善をお手伝いするTech Tipsシリーズ、CSTや外部の研究者によるリサーチウェビナー、特定のアプリケーションや疾患研究に関するビデオを視聴できます。

Dr. Reuben Shaw discusses advances in AMPK and autophagy signaling, identification and characterization of novel AMPK substrates including ULK1/Atg1, and the potential for ULK1 inhibitors in cancer therapeutics.
AMPKとオートファジーシグナル伝達の新展開
Watch this webinar to learn about a streamlined workflow for generation of CD19 CAR-T cells using BioTechne analytical instrumentation and anti-CAR linker antibodies from CST.
CAR-T細胞シグナル伝達と活性化を評価するための解析ツール
Watch this webinar to learn how the new CAR linker antibodies can interrogate the expression of an entire panel of CARs.
CAR-T細胞の特性解析用の新たな抗体
Understanding the functional mechanisms and signaling pathways that regulate activation of specific T cell subpopulations is crucial to optimizing effectiveness of the next generation of CAR-T therapies. Presented by Wendell Lim and Stanley Riddell.
CAR-T細胞を読み解く：次世代の免疫療法を推進する機能メカニズムの理解
1.ChIP和ChIP seq的区别和应用范围； 2.ChIP和ChIP seq 实验的操作步骤和成功要素； 3.酶解和超声的优缺点及如何正确选择； 4.如何选择合适的用于ChIP和ChIP seq的抗体； 5.CST 标准 DNA文库建立方法及ChIP-Seq测序数据分析解读
ChIP & ChIP-Seq实验原理与成功要素及NGS测序数据分析解读
The success of your ChIP experiment depends on the fragmentation of chromatin, a critical step in the ChIP protocol. This can be accomplished with either sonication or enzymatic digestion. But how do you decide which chromatin fragmentation protocol to use in your ChIP experiments? This video will show you the way.
ChIPにおける酵素法とソニケーション法の選択
To help cancer researchers to push their field forward, CST scientists dig into the literature to identify unmet needs. Dr. Cigall Kadoch shares her thoughts on what makes CST antibody development and validation unique in this video.
Cigall Kadoch博士：なぜCSTは独創的なのか
As the importance of the mammalian SWI/SNF (BAF) complexes in contributing to cancer became apparent, Dr. Kadoch realized there was a real lack of reagents to effectively study this class of proteins.
Cigall Kadoch博士：発見のための特異的かつ高親和性の抗体
Cigall Kadoch PhD keeps a running list of targets to advance mechanistic studies of human disease. Here, Dr. Kadoch discusses the value of collaborating with CST to develop novel reagents to probe ATP-dependent chromatin remodeling complexes.
Cigall Kadoch博士：科学とはチームスポーツ
Advances in multiplex IHC using Opal and validated CST rabbit primary antibodies to reliably stain for 6 markers in a mouse model of breast cancer; using Akoya Bioscience's Phenoptics platform to perform quantitative analysis of tumor biology.
CSTが開発した抗体とOPAL IHC法を用いたマウス組織のマルチプレックス解析
Video: the updated CUT&RUN kit provides improved results, confirmed compatibility with fixed frozen cells, tissues, and primary cells, uses even fewer cells.
CSTのCUT&RUNキット：柔軟性の検証
Our continuing goal is to make the highest quality, most consistent antibodies that work the first time, every time.
CSTの抗体検証
Know what to look for and dig deeper into the validation data for your antibody to avoid the frustration of ambiguous or misleading results.
CSTの抗体検証における戦略
Learn about the history of Cell Signaling Technology and our commitment to providing reproducible tools to move your science forward.
CSTの歴史
该视频重点介绍肿瘤微环境当中缺氧代谢与血管生成相关的调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标作为参考。
CST肿瘤微环境主题系列讲座---第一讲：缺氧代谢与血管生成
该视频将重点介绍与细胞骨架、细胞基质动态调控及EMT相关的主要调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标作为参考。
CST肿瘤微环境主题系列讲座---第三讲：细胞粘附、细胞骨架、细胞基质、EMT
该视频重点介绍肿瘤免疫微环境相关的主要参与细胞、调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标和新的研究方法作为参考。
CST肿瘤微环境主题系列讲座---第二讲：肿瘤免疫微环境
Learn how CUT&RUN can be performed in as little as 1-2 days using low cell number samples, and how CUT&RUN has been employed to study epigenetic remodeling and alternative splicing directed by the NF-κB pathway downstream of a viral oncoprotein.
CUT&RUN：エピジェネティックマークとメカニズムを解析するための新しい方法
This animated explainer shows how the CUT&RUN method works for mapping protein-DNA interactions in native chromatin, while using fewer cells and saving you time.
CUT&RUNによるクロマチンのプロファイリング
What antibodies can be used in a CUT&RUN protocol? What controls should be included to measure signal-to-noise? These and other frequently asked questions are answered in this edition of CST Tech Tips.
CUT&RUNのよくある質問トップ5
A comprehensive, step-by-step video of the CUT&RUN Protocol, from sample preparation to CUT&RUN DNA purification and qPCR quantification. Expert tips for critical steps and experimental design are highlighted to help you successfully complete the assay.
CUT&RUNプロトコール：​映像ガイド
A comprehensive, step-by-step video of the CUT&RUN DNA Library Prep Protocol for Illumina systems, picking up after the CUT&RUN protocol video. Key protocol steps and tips are highlighted.
CUT&RUN用のDNAライブラリー調製プロトコール：​映像ガイド
Watch this video to learn how CST® CUT&Tag kits, reagents, and antibodies fuel your research with consistent, reliable, and reproducible results.
CUT&Tagでクロマチンプロファイリングを迅速化
Meet Gary Kasof PhD, who over his career has developed some of the most-cited antibodies antibodies for studying cell death. Gary also shares advice for young scientists.
Gary Kasofに抗体の開発について聞く​
Learn how to boost sensitivity and reproducibility in protein detection to tackle low-abundance targets where traditional western blotting fails.
Going Beyond the Limits of Traditional Westerns
How sample type, processing steps, and characteristics of the target protein can impact detection and inform protocol optimization in immunofluorescence.
IFでお気に入りのタンパク質を検出するためのガイド
帮助科研工作者更好地理解 IHC 在疾病诊断中的核心作用，通过应用实例讨论 IHC 技术操作过程、结果分析中的细节和注意事项。介绍 mIHC 的原理方法，并以研究实例讨论 mIHC 在疾病研究中的价值。
IHC 和 mIHC 在疾病研究诊断中的方法介绍及注意事项
Discover advanced methods for studying TREM2 and downstream signaling in human-relevant iPSC-derived microglia to advance Alzheimer’s disease research.
Interrogating Cell Signaling Pathways in iPSC-Derived Microglia
Learn how InTraSeq™ Single Cell Analysis enables unbiased quantitation of RNA alongside intracellular and cell surface proteins at the single-cell level.
InTraSeq™シングルセル解析：RNAとタンパク質を同時に定量できる複合的なシングルセル解析手法で新たなシングルセル生物学を解明
By combining single cell analysis of intracellular protein phosphorylation states with RNAseq, InTraSeq technology provides deeper understanding of cellular heterogeneity and function.
InTraSeq™シングルセル解析：scRNAseqでより多くのデータを取得
This video describes the simplified PTMScan® HS workflow for LC-MS analysis of ubiquitination and SUMOylation that improves readout sensitivity, reduces sample prep time, and eliminates lyophilization, 9M urea, and antibody elution steps.
LC-MS解析用の高感度翻訳後修飾ペプチド濃縮
The CUT&RUN assay enables faster results from smaller samples to enable your analysis of protein-DNA interactions. Our CUT&RUN expert offers helpful protocol tips to get your CUT&RUN experiments running.
Learning Lab LIVE！CUT&RUNエキスパート、Fang Chen博士とのQ&A
Antibody optimization in pilot experiments is recommended prior to running full-scale immunohistochemistry assays.
Learning Lab LIVE！IHC専門家のJessica SimendingerとのQ&A
How does protein expression level factor in your ability to detect proteins and post-translational modifications by western blot?
Learning Lab LIVE！ウェスタンブロットエキスパート、Srikanth Subramanian博士とのQ&A
How can intracellular and extracellular protein readouts be combined in flow cytometry? This video offers protocol advice from our flow expert.
Learning Lab LIVE！フローサイトメトリーのエキスパート、Robert MacDonald博士とのQ&A
Why are antigen retrieval and antibody diluent important for the immunohistochemistry protocol? Our IHC expert offers protocol advice.
Learning Lab LIVE！免疫組織染色のエキスパート、Chris Grange氏とのQ&A
Need to choose fluorophores and antibodies when designing your immunofluorescence experiment? Our immunofluorescence expert has some advice.
Learning Lab LIVE！免疫蛍光染色のエキスパート、Virginia Bain博士とのQ&A
What to know about widefield versus confocal imaging settings for your microscope to get optimal immunofluorescence data. What to know about widefield versus confocal imaging settings for your microscope to get optimal immunofluorescence data.
Learning Lab LIVE！免疫蛍光染色のエキスパート、Virginia Bain博士とのイメージングのQ&A
Rapidly advancing single-cell and multi-omic technologies generate complex datasets, but how can they drive deeper insights and actionable discoveries?
Not All Antibodies Are Created Equal: Why Application‑Specific Validation Defines Single‑Cell Insights
To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition.
Phospho-Flowの習得：免疫細胞の活性化
This video introduces PTMScan technology, a platform for the enrichment of post-translational modifications (PTMs) in mass spectrometry-based proteomics studies.
PTMScan：翻訳後修飾のプロテオミクス
Dr. Reuben Shaw discusses the collaborative relationship his lab has developed with scientists at Cell Signaling Technology. This collaboration has led to the development and validation of substrate motif antibodies that has enabled profiling of the signaling activities in metabolic diseases and cancers in Dr. Shaw’s lab and around the world.
Reuben Shaw博士：CSTは成功のパートナー
Dr. Reuben Shaw provides his perspective as a cancer researcher on the value of developing research tools including modification-specific and phosphorylation-specific antibodies, in collaboration with Cell Signaling Technology, and how those tools enable the research community to push forward and make new discoveries.
Reuben Shaw博士：CSTは発見の燃料となる
To develop effective therapeutics and vaccines to fight the COVID-19 pandemic, solid experimental work using reliable, validated reagents and antibodies are as important as ever.
SARS-CoV-2研究にとって、抗体のバリデーションが極めて重要な理由
SignalStar® Multiplex IHC for spatial biology. Up to 8-plex protein detection generated in just 2 days. Watch the on-demand webinar.
SignalStar® Multiplex IHCは実験にかかる時間を最大70%短縮して探索を加速
Learn insider tips and tricks for successful SignalStar® Multiplex IHC experiments. This video covers critical steps, troubleshooting, and optimization for both manual and automated protocols to help you maximize experimental success and confidently interpret your results.
SignalStar®実験を成功させるためのヒントとコツ
This presentation recorded at SLAS describes the use of antibodies to probe targets in the TGF-β/Smad Signaling pathway using high throughput, automated quantitative ELISA and fluorescence-based microscopy assays.
SMADシグナル伝達経路を定量的に評価するためのマルチスケールアプローチ
本次研讨会将通过介绍CST Western Blot标准操作步骤，向您阐释WB操作中的关键点及注意事项，特别是影响最终结果呈现的样本制备和抗体孵育与检测。通过真实失败案例，如无信号、高背景、条带大小不正确、条带弯曲拖尾等，分析WB常见的问题及原因，并提供相应的解决策略。
WB标准操作流程与常见问题解答
Drs. Cominelli and Kugathasan describe new techniques to identify and target inflammatory signaling in gastrointestinal immune cells in this webinar.
あなたの腸に耳を傾けましょう：炎症性腸疾患における免疫細胞の役割を解明する
This presentation explores multidimensional, image-based methods for immunophenotyping of cancer immune microenvironments and the design of antibody panels for high-plex platforms.
イメージングマスサイトメトリーの最適化において考慮すべきこと
Need to decide between different lysis buffers for your Western Blot (WB)? Before you start running your proteins with RIPA buffer, cell lysis buffer, or SDS, Sarah has some questions you should consider.
ウェスタンブロッティング (WB) に用いる細胞溶解バッファーの選択
Western Blot Basic Webinar (JP)
ウェスタンブロッティング (WB) の基礎
What's the benefit of sonicating lysates before loading on a gel? If you don't perform sonication as part of your western blot workflow, you might want to check out this quick Tech Tip presented by Srikanth.
ウェスタンブロッティング (WB) 用ライセートのソニケーション
In this video, our experts provide protocol guidance for lysate preparation steps prior to protein electrophoresis and western blotting.
ウェスタンブロッティングの結果に溶解バッファーやソニケーションが与える影響
Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. We understand that western blotting experiments are time consuming and critical to your research progress. For that reason, we provide optimized protocols along with reference information and technical support to make your western blotting experience successful.
ウェスタンブロッティングプロトコール
Western Blotting Protocol (Japanese Audio)
ウェスタンブロッティングプロトコール (日本語音声)
Western Blotting Protocol (CN)
ウェスタンブロッティングプロトコール 蛋白印迹实验步骤
Western Blot Troubleshooting Guide (Japanese Audio)
ウェスタンブロット (WB) のトラブルシューティングガイド (日本語音声)
Lysate controls for Western Blot (WB) experiments are from a sample with known expression of your protein of interest, giving you an idea of how much signal to expect from your experimental samples. In this Tech Tip, Alex discusses how to use positive lysate controls, how they differ from loading controls, and considerations for phospho-specific antibodies.
ウェスタンブロット (WB) の実験デザイン：ポジティブコントロールの選択
Western Blot Troubleshooting Guide (CN)
ウェスタンブロットトラブルシューティングガイド 实验操作步骤视频解析
Watch this video to learn how epitope tags can be leveraged in your experiments when your protein of interest is newly discovered, expressed at low abundance, or poorly immunogenic.
ウェスタンブロットと免疫沈降におけるエピトープタグの利用
Common problems encountered in western blotting include low signal or high background. This video helps you troubleshoot problems in your Western Blot experiment, tips to help diagnose problems, and provides solutions to ensure you get the expected results in the shortest amount of time.
ウェスタンブロットのトラブルシューティングガイド
Multiplex immunohistochemistry (mIHC) supports detection and spatial characterization of multiple (>6) proteins in preserved tissue. Learn how mIHC enables tumor immunology and immune checkpoint studies.
ウェビナー - がん免疫研究ツールとしてのmIHCと新規モノクローナル抗体
Why is antibody validation important? How can vendors, researchers, and journals improve the reproducibility of research? This webinar addresses these questions and highlights considerations for selecting antibodies and designing experiments.
ウェビナー - 再現性の実現：科学研究における信頼性の高い抗体
The reproducibility of scientific research is a rising concern in both academia and industry. This webinar will highlight how antibody specificity affects reproducibility in biomedical research and identify key requirements for proper antibody validation.
ウェビナー - 抗体検証ラウンドテーブル：特異性 + 感度 + 再現性 = 検証
This webinar discusses the application of both traditional IHC and new tissue processing methods in transgenic mouse models of Alzheimer’s disease. These methods enable whole-brain analysis and insights into molecular and neural circuit mechanisms underlying Alzheimer’s disease.
ウェビナー - 神経変性疾患研究のための抗体染色
Uncovering the mechanisms underlying RNA modifications and RBP function are emerging frontiers in medicine that take the study of gene regulation a step beyond epigenetics
エピトランスクリプトミクスを読み解く：疾患におけるRNA修飾とRNA結合タンパク質の重要性
Learn how phosphorylation, ubiquitination, & Atg8ylation impact autophagy, a key cellular process linked to health & disease. Watch the on-demand webinar featuring Reuben Shaw & Vojo Deretic.
オートファジーの隠れたドライバー：翻訳後修飾の役割の探索
Drs. Schietinger and Youngblood review the underlying mechanisms and epigenetic programs determining tumor-specific T-cell dysfunction and reprogrammability and discuss current challenges and outstanding research questions in cancer immunotherapy.
がんにおける免疫応答のエピジェネティック制御を解読する
This webinar will explore the idea that a better understanding of developmental biology signaling pathways will advance our understanding of adult tumors and cancer stem cells as well as boost our ability to create effective therapeutics to fight a broad array of cancers.
がんの重要なシグナル伝達経路 - 発生生物学との関連性
Accelerated metabolism is a hallmark of both immune responses and cancer. This webinar describes the roles of aerobic glycolysis, lipid metabolism, and mitochondrial remodeling in immune metabolism, and explains how metabolic changes impact innate immunity.
がんの重要なシグナル伝達経路：免疫代謝の最新の動向
This webinar will examine how, by targeting proteins responsible for the crosstalk between epigenetics and metabolism, we may be able to develop new and effective therapeutic options for cancer treatment.
がんを解読する：エピジェネティクスと代謝、腫瘍形成の交差
Tumor heterogeneity is a major clinical challenge and is driven by DNA damage and genomic instability. This webinar describes several mechanisms of tumorigenesis caused by DNA damage, including changes to copy number, and discusses combinatorial therapeutics that increase replication stress in order to halt cancer cell division and tumor growth.
がんを解読する：がんにおけるゲノムの不安定性
This webinar will focus on how therapeutic interventions targeting dendritic cells can promote tumor immunity and enhance the efficacy of cancer therapy.
がん免疫の解読：がんにおいて自然免疫を標的にする
Key signaling pathways modulate immune cell activation and tumor checkpoint evasion. Improved understanding of tumor microenvironment crosstalk and mechanisms of response/reistance will be key for next-generation cancer immunotherapies.
がん免疫の解読：がん免疫応答の細胞機構を標的化する
Exploring the plasticity of tumor-associated macrophages (TAMs) and challenges in distinguishing M1- versus M2-polarized macrophages.
がん微小環境におけるマクロファージ研究の新展開
This webinar explores how aberrant histone modifications can affect the expression of key genes and contribute to the pathological progression of cancer. Specific epigenetic pathways are candidates for therapeutic intervention.
がん経路の標的化：エピジェネティクスに関する疑問
This webinar explores how cancer cells reprogram their metabolic pathways to increase energy production. The roles of metabolic changes, autophagy, and cell death in influencing tumor progression, and potential therapeutic interventions are considered.
がん経路の標的化：がんの代謝と増殖
The abnormal tumor microenvironment fuels oncogenic progression and treatment resistance. By better understanding stromal/tumor interactions and remodeling of surrounding matrix and blood vessels, we can develop more effective treatment options, including immunotherapy.
がん経路の標的化：がんの微小環境
Some cancers develop resistance to drugs that target signaling pathways and become increasingly aggressive. By understanding how cancer cells re-wire their signaling, more effective clinical strategies leveraging new and existing therapies can be designed.
がん経路の標的化：がんの治療への抵抗性
Tumors exploit immune checkpoint pathways to evade detection and destruction by the immune system. Learn how immunotherapy re-activates the immune system to kill cancer cells by targeting PD-1 / PD-L1, CTLA-4, and other targets.
がん経路の標的化：免疫チェックポイントを理解する
Learn how the optimal crosslinking time in Chromatin Immunoprecipitation (ChIP) can be influenced by sample type, and by target protein (histone, transcription factor, or cofactor). Longer crosslinking time is beneficial in certain situations, but over-crosslinking can make it harder to shear genomic DNA. Fang breaks it down in this Tech Tip.
クロマチンのクロスリンクに 適切な時間は？ クロマチン免疫沈降 (ChIP)
Chromatin fragmentation can be performed using either sonication or enzymatic digestion. This Tech Tip video covers the sonication protocol, in which sonication facilitates both cell and nuclear lysis as well as fragmentation of the chromatin. Varying the sonication time affects target enrichment and chromatin yield.
クロマチンのソニケーション：ソニケーション法クロマチン免疫沈降 (ChIP) プロトコール
Chromatin fragmentation can be performed using either sonication or enzymatic digestion. Despite its name, the enzymatic protocol still requires brief sonication. In this Tech Tip, Fang shows you what to look for to ensure sufficient chromatin yield.
クロマチンのソニケーション：酵素法クロマチン免疫沈降 (ChIP) プロトコール
More antibody isn't always better! The optimal amount of antibody to use in a ChIP assay varies, depending on the antibody's affinity, sensitivity and specificity. See how the amount of antibody used can affect your Chromatin Immunoprecipitation (ChIP) results in this Tech Tip featuring Fang.
クロマチン免疫沈降 (ChIP) アッセイに使用すべき抗体の量
This presentation gives an overview of the chromatin immunoprecipitation (ChIP) technique and three keys to success: optimized protocol & quality reagents, chromatin crosslinking & fragmentation, and validation.
クロマチン免疫沈降 (ChIP) アッセイの成功のためのガイド
The goal of this video is to guide you through chromatin fragmentation, a critical step in a Chromatin Immunoprecipitation (ChIP) experiment. It is important to consider whether the target protein is a histone modification, transcription factor, or transcription co-factor since each protein binds differently to DNA. Due to these binding differences, additional measures should be taken into account when optimizing chromatin fragmentation for each protein class, whether performing sonication or enzymatic digestion.
クロマチン免疫沈降 (ChIP) においてクロマチンの断片化を最適化する方法
ChIP Basic Webinar (JP)
クロマチン免疫沈降 (ChIP) の基礎
The success of your ChIP experiment relies on the antibody you choose. This video describes the different validation approaches our scientists use to ensure the antibodies we recommend for ChIP-qPCR and ChIP-seq will perform as you expect.
クロマチン免疫沈降 (ChIP) の結果を信頼できますか？
Drs. Benjamin Wolozin and Dorothee Dormann present recent research on phase separation mechanisms regulating stress granule proteins implicated in ALS, frontotemporal dementia, and other neurodegenerative diseases.
ストレス顆粒：神経変性との関連性を解読
Unlock Th17 cellular mechanisms with InTraSeq single-cell analysis. Watch the webinar to learn about RNA & protein co-quantification & more.
すべてのデータを取得可能：病原性Th17細胞と非病原性Th17細胞の分子メカニズムの解明
Dr. Nathanael Gray of the Stanford Cancer Institute discusses efforts to synthesize and characterize small molecule degraders of cancer targets, including kinases and transcriptional regulators.
タンパク質分解に関する課題と可能性
Targeted protein degradation selectively alters protein abundance by co-opting the cellular degradation machinery. The degradation tag (dTAG) system can be used for target validation and mechanistic studies.
タンパク質分解の新たな展望：dTAGシステムを利用した迅速で選択的なタンパク質の制御
This video provides a step-by-step guide to the immunohistochemistry protocol for paraffin-embedded tissue tissue sections, used by scientists in Cell Signaling Technology's IHC group.
パラフィン包埋組織切片の免疫組織化学染色 (IHC) プロトコール
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (Japanese Audio)
パラフィン包埋組織切片の免疫組織化学染色 (IHC) プロトコール (日本語音声)
This video describes the application of robust multiplex imaging technologies to interrogate myeloid and lymphoid lineages to provide spatial insights of many cell types in the tumor microenvironment.
ヒトがん組織の微小環境における、腫瘍と免疫系細胞の空間的配置の捕捉
Speakers discuss the assembly and biological architecture of mammalian SWI/SNF complexes and the role of cancer-specific SWI/SNF complex mutations (present in 20% of cancers) in driving oncogenicity. Also, novel approaches toward the identification of small molecule therapeutics for this class of human tumors and the development and validation of new high quality recombinant monoclonal antibodies for ChIP and ChIP-seq. And finally, the important factors to consider for optimizing different types of proteins—including histones, transcription factors, and cofactors—for ChIP.
ヒト疾患におけるエピジェネティクス制御因子の構造と機能
Webinar about Post-translational Modification Enrichment and Quantitation for Precision Medicine and Drug Development
プレシジョンメディシン (精密医療) および医薬品の開発における翻訳後修飾 (PTM)の濃縮と定量
This webinar provides practical advice to optimize flow cytometry protocols to reveal essential signaling insights needed to uncover the activity and function of rare immune cell subsets.
フローサイトメトリー：免疫細胞のシグナル伝達カスケードで表現型と機能を明らかにする
Selecting antibodies and a protocol for flow cytometry comes with several considerations which are discussed in this webinar.
フローサイトメトリー：抗体染色の手引き
Should you switch from western blot to flow? We’ll go over situations where flow cytometry can take your research further in this Tech Tip video with Sarah.
フローサイトメトリーが役立つ4つの状況
If you’re only looking at surface markers in your flow cytometry, you’re missing out! We’ll discuss several protocol approaches to multiplexing so that you can analyze both surface markers and intracellular targets.
フローサイトメトリーで細胞表面と細胞内の標的を組み合わせる方法
Adding intracellular signaling targets to your flow cytometry analysis? You'll need to fix and permeabilize your cells to allow antibodies to access their epitopes. Rob explains the protocol variations.
フローサイトメトリーにおいて細胞を固定し透過化する方法
Combining analysis of surface markers and intracellular signaling readouts through multiplexed flow cytometry adds power to your experiments. This can present challenges when designing antibody panels. This video walks through an example experiment to illustrate how antibodies can be selected to assess activation of T cell subpopulations.
フローサイトメトリーによる免疫応答の解析
In this video, our flow expert compares different cell dissociation methods and how to assess their effects on cell viability, protein expression, and signaling.
フローサイトメトリーの準備：細胞解離法
Watch this 3-D animation to see how flow cytometry enables the simultaneous characterization of intracellular signaling states and phenotyping of rare cell populations in a heterogeneous sample.
フローサイトメトリー入門
Webinar on mass spectometry used to investigate intracellular signaling activities via antibody enrichment of post-translational modifications
プロテオミクスとシグナル伝達
An overview of oh mass spectrometry-based proteomics studies can be a powerful tool for drug discovery and development
プロテオミクスに着目した、創薬パイプラインに役立つ生物学の解明
The complexity of the proteome makes discovery research seem like trying to find a needle in a haystack. Learn how enrichment with PTMScan decreases sample complexity and increases the likelihood that you'll find what you're looking for.
プロテオミクスの可能性：発見への明確な筋道 (パートI)
Learn how PTMScan technology facilitates translational discovery, identification of disease drivers, and development of markers and treatments, as exemplified in the study of non-small cell lung cancer (NSCLC).
プロテオミクスの可能性：発見への明確な筋道 (パートII)
This video illustrates how the CD133 (D4W4N) XP® Rabbit mAb was validated by CST. The D4W4N clone is approved for western blotting and IHC, and detects human CD133 protein with exceptional specificity and sensitivity. It outperforms the currently available CD133 antibodies in market, setting a new industry standard for the detection of CD133 protein.
ポスター - がん幹細胞マーカーCD133を標的とするグリコシル化非依存性抗体の検証
Highly Multiplexed IHC Assays to Examine Immune Checkpoints and Biomarkers for Immunotherapy
ポスター - 高度なマルチプレックスIHCを用いた免疫チェックポイントと免疫療法のバイオマーカーの解析
Quantitative analysis of the SMAD signaling pathway using ELISA, high content imaging, and high-resolution confocal approaches were used to study the regulation of angiogenesis and cell proliferation.
ポスター：SMADシグナル伝達経路を定量的に評価するためのマルチスケール解析
Optimizing the order of serial antibody labeling steps for fluorescent multiplex IHC can help you achieve a balanced set of signals. This Tech Tip video shows how to evaluate epitope sensitivity to guide antibody panel construction.
マルチプレックスIHCの最適化：抗体の染色順序
When designing an antibody panel for multiplex IHC, it’s important to optimize each antibody’s dilution and which fluorophore it’s paired with. This will help your staining achieve a balanced set of signals.
マルチプレックスIHCの最適化：抗体の組み合わせとタイトレーション
The CST protocol for Fluorescent Multiplex Immunohistochemistry allows you to characterize 6 or more targets in one sample with flexible experimental design by employing a serial labeling strategy. In this video, Jen highlights five key protocol steps you can optimize to help get a balanced set of signals.
マルチプレックス免疫組織化学染色 (IHC) の最適化を始めるための5つのヒント
Drs. Michael Heneka and Christian Haass present recent findings on how signaling involving TREM2 in microglia triggers the innate immune response in neurodegenerative diseases including Alzheimer's.
ミクログリアを解読する：神経変性疾患における細胞経路の探究
Good IHC protocols provide the proper conditions for a specific binding event to occur. It is therefore essential that you calibrate the ionic strength and pH of the antibody diluent to the specific antigen-epitope interaction you are trying to detect.
より良いIHC ステップ2：抗体の希釈
Detection reagents bind to the primary antibody, either directly or indirectly, through a secondary antibody intermediate. When a chromogenic substate is introduced, a precipitate forms that deposits at the site of the primary antibody antigen binding event.
より良いIHC　ステップ3：検出試薬
In designing your experiment, choose a chromogen that produces a color with appropriate contrast to your counterstain, and of a high enough intensity to reveal the antigen you are trying to detect.
より良いIHC　ステップ4：発色基質
Watch this video to learn how two different methods (antibody-based immunoaffinty enrichment and IMAC) combine for improved coverage of the phosphoproteome.
リン酸化ペプチド濃縮のための相補的な技術
This video highlights the role of histone modifications and epigenetic regulation in driving progression of breast cancer. Targets include, Estrogen Receptor, Ezh2, UTX, and HK27Me3.
乳がんのエピジェネティックドライバー
Understanding the activity of intracellular signaling pathways are crucial to developing therapies that maximize efficacy and minimize toxicity. Mass spectroscopy-based proteomics and intracellular flow cytometry can further our understanding of immune regulation in cancer and improve therapeutic potentials.
免疫制御を学ぶ：グローバルなリン酸化のプロファイリングとフローサイトメトリー
Protein enrichment by immunoprecipitation is frequently carried out prior to analysis. In this Tech Tip, Sarah presents considerations for choosing antibodies and beads for IP.
免疫沈降に用いる抗体の選び方
Watch this video for a step-by-step demonstration of the Immunoprecipitation Protocol for Native Protein for Western Blot analysis. Includes guidance for experimental design, choosing antibodies and beads, and tips and tricks for successful protein IP.
免疫沈降プロトコール：​映像ガイド
Dr. David Rimm of Yale School of Medicine discusses multiplexing spatial imaging technologies and their development to improve response prediction for immunotherapy. This webinar was recorded at AACR 2022.
免疫療法でより高い効果が期待される新しい候補因子の発見につながる空間的な画像解析手法
Researchers have been investigating several potential avenues to counter tumor immunosuppression and re-activate the immune system. One of these is CAR-T therapy, which is designed to harness the potent cytotoxicity of T cells by re-engineering their surface receptors. This video gives an overview of this exciting field. What’s on the horizon for the CAR-T therapy field, and what challenges are researchers and clinicians going to tackle to enable the next success? We’ll explore this exciting frontier in part two of our CAR-T video series.
免疫療法とCAR-T細胞
Dr. Gygi describes Bioplex, a project to systematically map protein interactions. Knowing these interactions can provide insight into a protein's biological function, place it within signaling networks, and generate mechanisms and hypotheses underlying biological processes.
免疫精製実験から明らかになるタンパク質相互作用ネットワーク
Visualize immune cell identity, function, and spatial context with CST’s validated IHC antibodies. Maximize your research impact with precision tools.
免疫細胞の表現型と機能の解析
In this presentation, we'll cover different IHC controls to determine if your observed staining (or lack thereof) is accurate and the antibody is specific.
免疫組織化学染色 (IHC) におけるコントロールの重要性
Learn how choice of antibody diluent can affect your IHC results in this Tech Tip with Chris. Differences in antibody diluent properties can influence antibody-epitope interactions and specificity, and ultimately, the strength of your staining.
免疫組織化学染色 (IHC) における抗体希釈液の影響
Antigen retrieval can influence the strength of staining obtained in IHC. In this Tech Tip, Franny and Abigail show you why antigen retrieval (AR) is important for immunohistochemistry (IHC), and give you tips on how to set up Heat Induced Epitope Retreival in your microwave.
免疫組織化学染色 (IHC) における抗原賦活化 (AR) の方法
How do you know if your antibody is working properly in immunohistochemistry (IHC)? In this Tech Tip, Abbey discusses the use of positive and negative tissue controls, isotype controls, and controls for phospho-specific and modification-specific antibodies.
免疫組織化学染色 (IHC) に用いる様々なコントロール
When setting up Immunohistochemistry (IHC) experiments, cell pellet controls can be used to test antibody specificity and tell you whether your protocol is working or needs to be adjusted. In this Tech Tip, Chris highlights situations where cell pellet controls can be valuable.
免疫組織化学染色 (IHC) に細胞ペレットコントロールを組み込む方法
Immunohistochemistry Basic Webinar (JP)
免疫組織化学染色 (IHC) の基礎
You probably know that a highly specific, high affinity primary antibody is key to a successful immunohistochemistry. Companion reagents such as buffers are just as important. We will describe an example of our experience with protocol optimization and highlight the role of Antigen Retrieval for IHC.
免疫組織化学染色 (IHC) の改善　概要・ステップ1：抗原賦活化
How does antibody validation affect your IHC results? Learn about the approaches scientists at CST use to validate antibodies for IHC in this video: cell pellets, multiple tissues, and treatments with activators, inhibitors, or blocking peptides.
免疫組織化学染色 (IHC) の結果を信頼できますか？
This video highlights considerations for optimizing antibodies and adjusting the protocols when transfering protocols from manual to automated immunohistochemistry.
免疫組織化学染色 (IHC) プロトコールの自動染色装置への移行に関するQ&A
Explore the difference between two major types of fixatives for immunofluorescence: crosslinking aldehydes including formaldehyde, and alcohols including methanol. John describes how choice of fixative can impact the structure of epitopes and the fluorescent properties of the sample itself, and affect your results.
免疫蛍光染色 (IF) におけるホルムアルデヒド固定とアルコール固定
Immunofluorescence Basic Webinar (JP)
免疫蛍光染色 (IF) の基礎
Before you start your next immunofluorescence (IF) experiment, ask these three questions to ensure you will get reliable results and avoid headaches: 1) Is your antibody specific? 2) Is your antibody supported by an optimized IF protocol? 3) Is your antibody performing consistently?
免疫蛍光染色 (IF) を始める前にすべき3つの質問
Microglia, critical mediators of neuroinflammation, are shown in the context of a whole mouse brain by probing with anti-Iba1/AIF1.
全組織が透明化されたマウス脳におけるミクログリアの可視化
Watch this webinar for applications, tips, and tricks for Simple Western, for fast, reliable, and hands-free automated western blotting using CST antibodies. See an overview of the CST-Bio-Techne partership, brining westerns into the 21st century.
全自動Simple Westernでウェスタンブロットを現代化 - お客様の声
This video explains the roles of the bait and prey proteins in co-IP, and applications of immunoprecipitation for studying protein-nucleic acid interactions.
共免疫沈降 (co-IP) のBaitとPrey、免疫沈降 (IP) のその他アプリケーション
本讲座将为大家系统地介绍cGAS-STING信号通路在天然免疫和肿瘤免疫研究领域的广泛作用，以及作为干预靶标的巨大潜力。
具有巨大潜力的天然免疫和肿瘤免疫干预靶标——cGAS-STING信号通路
This video presents tips to help your immunoprecipitation experiment: how to avoid IgG masking; optimizing epitope tags; experimental design (controls); what to consider when choosing lysis buffers, antibodies, and beads.
内因性およびタグ付加タンパク質モデルを使用した、共免疫沈降によるタンパク質相互作用の検証
This video describes considerations for optimizing capture and detection antibody pairs to enable consistent ELISA results.
最適化されたELISAで信頼できる結果を取得
本场讲座将逐一介绍多种细胞程序性死亡（凋亡、自噬、程序性坏死、细胞焦亡）相关信号通路关键蛋白和研究策略，并就多种细胞程序性死亡形式之间的联系和转化展开讨论，为大家全面认识细胞程序性死亡和开展系统研究提供参考。
凋亡相关信号通路研究进展和关键蛋白研究策略
Epigenetic regulators contribute to the progression of prostate cancer through altered androgen receptor signaling, as well as other mechanisms. This video introduces three epigenetic targets: Androgen Receptor, Glucocorticoid Receptor, and Ezh2.
前立腺がんのエピジェネティックドライバー
To keep your projects moving forward, you need to count on reagents that will work as expected and be available throughout the lifetime of your study.
創薬パイプラインを保護する
This webinar will focus on the utility of the PTMScan method in early drug discovery and the future of automation in multiplexed analysis of cellular signaling.
創薬早期における翻訳後修飾 (PTM) のプロテオミクスプロファイリング
Imaging Mass Cytometry (IMC) is a novel technique used to visualize over 50 antibodies simultaneously on tissues with sub-cellular resolution. This webinar will reveiw this technique and how antibody quality impacts highly multiplexed techniques.
単一細胞の高度な多重解析 (マスサイトメトリー) による腫瘍不均一性の時空間的解析
Watch this 20-minute webinar for tips and tools to optimize simultaneous incubation of different antibodies, plus insignts into maximing multiplexing efficiency while maintaining assay quality.
単純化された染色：フローサイトメトリーによる不均一な集団のシグナル伝達の解析
本讲座全面讲解如何深入开展信号通路的研究，从大数据挖掘到锁定目标信号通路，进一步探讨疾病分子机制，并涉及到相关主流的实验技术，如修饰蛋白质组数据库使用、WB、ChIP、IHC、PTMScan蛋白质翻译后修饰高通量筛选技术等。
如何让你的发现可靠可重复？千里之行始于足下
Designing or troubleshooting flow cytometry experiments to analyze intracellular signaling? Here’s what you need to know.
実験のデザイン：フローサイトメトリーによる細胞内シグナル伝達の解析
Good controls can help you get your experiment back on track if something goes wrong, and can help you have confidence in your results. In this Tech Tip video, Ginny goes over three types of controls for your immunofluorescence (IF) experiment: positive controls, endogenous controls, and negative controls.
実験のデザイン：免疫蛍光染色 (IF) におけるコントロールの設定
Answers to common questions about antibody validation and the roles of both the vendors and the scientists who use them in their research.
抗体の検証Q&A
CST has always believed that the only way to produce and validate our products is to do it in-house. That way, we can guarantee that they are rooted in sound science, and that our antibodies will be specific and reproducible tools available to research scientists.
抗体の物語 - ホワイトボード版
作为指导方针，本次讲座将与您分享CST流式细胞术专家在日常的试验工作中经常使用的经验和技巧。
掌控Phospho-Flow： 巧用细胞内、外流式细胞术优化免疫细胞活化分析
This video demonstrates the technique used for splitting adherent cell lines with trypsinization.
接着細胞株の分割方法：ステップごとの実演映像
By selectively targeting previously inaccessible oncogenic proteins for degradation, researchers are enabling functional studies with superior kinetic resolution and discovering new therapeutic targets.
標的タンパク質分解：がん治療薬の開発を促進する、治療標的の制御と評価
Is the performance an unconjugated antibody enough to validate a conjugated version of the same clone for your assay? Not necessarily! Learn why in this video.
標識抗体：知っておくべきこと
Innovative anti-linker monoclonal antibodies from CST eliminate the need to develop unique detection reagents for every new chimeric antigen receptor (CAR) molecule developed, speeding detection in flow cytometry panels.
汎用性のある画期的な抗体を用いたCARの発現の検出
How to combine modulators with selection of antibody targets and controls in western blot experiments.
活性化剤と阻害剤を用いたウェスタンブロット実験におけるシグナル伝達活性の調節
随着流式细胞术近几年的飞速发展，它已成为生物科学领域最有力的分析技术之一。对于这一技术，有的人却望尘莫及，问题层出不穷，比如，胞内染色染不出，找不到合适的抗体，修饰蛋白不知道怎么刺激等等。其实，跳出墨守陈规的流式应用，深度挖掘更深层次的流式实验，助力科研及临床的发展，是所有 Flower 为之奋斗的目标。本讲座将带您了解 Cell Signaling Technology (CST) 流式产品在细胞通路研究中的独到之处，让我们一起在广阔的流式世界中，携手同行，共辟蹊径。
流式 (Flow) 入通路，蹊径何处寻 | Webinar
This video demonstrates the technique used for splitting suspension cell lines.
浮遊細胞株の分割方法：ステップごとの実演映像
Reproducibility Issues in Life Science Research: Antibody Validation Challenges
生命科学研究における再現性をめぐる問題：抗体検証の挑戦
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (CN)
用于石蜡包埋的组织切片的免疫组织化学实验步骤 | Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections
This video highlights epigenetic regulators that contribute to progression of these blood cancers: mixed lineage leukemia, myelodysplastic syndrome, and acute myeloid leukemia. Here are several targets involved in epigenetics to consider for your MLL, MDS, or AML research: MLL1, p300/CBP, DOT1L, DNMT3A, TET2, and 5-methylcytosine (5-MC).
白血病のエピジェネティックドライバー
Drs. Michael Keebler and Chaolin Zhang present work on protein-RNA dynamics in neuronal synapses and RNA splice regulation in neuronal development.
神経の発達と疾患におけるRNA結合タンパク質の役割を解読
This webinar will focus on the potential role of innate immunity in neurodegeneration and cognitive function, particularly in Alzheimer's disease.
神経変性の解析：炎症、免疫応答、アルツハイマー病
This video shows how you can amplify up to 8 targets in mouse or human tissue in just 2 days, with flexible panel design and high-quality antibodies from CST.
空間生物学の新たな主役：SignalStar® Multiplex IHC
This video highlights three essential tips for splitting mammalian cell cultures, and explains how to use ratios or a cell counter to when propagating cells.
細胞の分割：その重要性と3つの不可欠なヒント
Reliably identify senescence signaling in your samples using biomarkers with a 3-stage approach outlined in this video. No single assay is sufficient, but screening, co-staining, and verification with multiple biomarkers can improve your confidence.
細胞や組織で細胞老化を特定する方法
Drs. Gary Schwartz and J. Julian Blow discuss advances in understanding CDKs and how CDK inhibitors can be used in combination with cancer therapeutics.
細胞周期の解読：がんの細胞周期調節の役割
Keep your cell and tissue culture free of contamination - watch this how-to video for tips and advice from our scientists.
細胞培養：無菌/滅菌操作
Studies of neurodegeneration using mouse models of Alzheimer's disease can now employ whole-brain tissue clearing techniques to better visualize expression of β-Amyloid and other hallmarks of disease.
組織透明化技術によるβ-アミロイド染色の全脳イメージング
Understanding the underlying biology of fibrosis is critical for the diagnosis, treatment, and management of cardiac fibrosis, nonalcoholic steatohepatitis (NASH), and other fibrotic diseases
線維症を読み解く：肝臓と心臓の線維性疾患の原因
本讲座将概述细胞代谢相关的信号通路，并举例说明系列重要细胞代谢研究靶点，和精彩研究案例思路
细胞代谢相关信号通路概览，重要靶点和研究案例分享
细胞焦亡与其他多种细胞程序性死亡形式之间存在相互联系和转化，共同构成细胞程序性死亡的复杂体系，维持细胞和机体的内稳态。
细胞焦亡信号通路的关键蛋白和研究动态
细胞程序性死亡(Programmed Cell Death, PCD)，是指细胞接受某种信号或受到某些因素刺激后，为了维持内环境稳定而发生的一种主动性消亡过程。它
细胞程序性死亡相关信号通路关键蛋白和研究策略
PTMScan® from CST uses the K-e-GG ubiquitin remnant motif antibody, applying it to perform large-scale quantitative analysis of the ubiquitylome.
翻訳後修飾 (PTM)：質量分析をベースとするプロテオミクス用の抗体の濃縮
This webinar will focus on the use of simultaneous affinity enrichment workflows by combining different PTM antibodies for ‘one-pot enrichment’ approaches
翻訳後修飾をデータに依存せずに取得する：課題と機会
Accumulation of senescent cells is considered a hallmark of aging and can drive many age-related pathologies including dementias, arthritis, atherosclerosis, and others.
老化を読み解く：老化をDNA障害と細胞周期に関連付ける
Learn how spatial biology and multiplex IHC can be used to understand the immune system's role in disease and therapy. This webinar covers assay design, antibody selection, and methods for various plex sizes.
背景が重要：免疫学および免疫療法における空間生物学の活用
How does choice of blocking protein - nonfat dry milk vs bovine serum albumen (BSA) - affect your Western Blot (WB) results? In this Tech Tip, Srikanth explains our protocol recommendations to reduce background caused by nonspecific binding.
脱脂粉乳かBSAか？ ウェスタンブロッティング (WB) でのブロッキングタンパク質の選択
该视频将将逐一介绍参与自噬信号通路的关键分子，包括ULK1 、Beclin1、LC3、Atg5，ATG12，P62等
自噬相关信号通路关键蛋白和研究策略
本场 Webinar 详细介绍 PTMScan® 技术，并结合精准医学中的应用案例介绍如何选择和使用 PTMScan® 相关产品。
蛋白翻译后修饰组学技术 (PTMScan®) 在精准医学中的应用
This presentation showcases the use of antibodies immunofluorescence and flow cytometry, and the importance of validation.
蛍光イメージングとフローサイトメトリーによる細胞シグナル伝達の解析
Does your immunofluorescence (IF) look like a bright mess? In this edition of Tech Tips, Ginny talks about what too much signal means, potential causes, and solutions for this problem.
蛍光免疫染色 (IF) のトラブルシューティング：明るいシグナルが過剰な場合
To enable surface characterization of chimeric antigen receptors (CARs), scientists at CST developed versatile and specific antibodies that detect the linker sequence of scFv domains. Watch the poster presentation from AACR.
表面発現したscFv融合CARの検出のための抗リンカーモノクローナル抗体の生成と検証
Drug discovery and development can incorporate proteomics studies which enable accelerated target identification and deeper insights of mechanism of action and off-target effects.
迅速な創薬のためのカスタム・プロテオミクスソリューション
Optimizing chromatin fragmentation can impact the quality of ChIP-qPCR and ChIP-seq results. This video provides guidance on choosing a fragmentation protocol, ChIP-seq library QC, and troubleshooting ChIP-qPCR results.
適切なChIPプロトコールの選択と信頼性の高いデータを得るための方法
Infiltrating myeloid cells are analyzed in the tumor microenvironment using multiplex IHC, employing new monoclonal antibodies to IDO1 and Arginase.
骨髄性免疫抑制の機構：シングルおよびマルチプレックスIHCを用いた、腫瘍微小環境の機能的キャラクタリゼーション
The chromatin immunoprecipitation (ChIP) assay is a powerful technique used for probing protein-DNA interactions in studies of chromatin regulation and epigenetics. This webinar discusses important factors to consider when performing ChIP, including use of highly validated antibodies, optimized protocols and reagents, and the advantages of using enzyme-based chromatin digestion.
高度に検証された抗体と最適化されたChIPアッセイによるエピジェネティクス解析