2’,3’-cGAMP (sodium salt) #35573

Product Usage Information

2’,3’-cGAMP (sodium salt) is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 500 μg of powder in 139 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, continue to store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight: 718.4 g/mol

Purity: >98%

Molecular Formula: C₂₀H₂₂N₁₀Na₂O₁₃P₂

CAS: 1441190-66-4

Solubility: Soluble in DMSO at 50 mg/ml and water at 10 mg/ml.

Background

2’,3’-cGAMP (sodium salt), also known as 2’,3’-cyclic-GMP-AMP, is a second messenger produced by the enzyme cGAMP synthase (cGAS) in response to cytoplasmic DNA (1,2). cGAMP binds and activates the stimulator of interferon genes (STING), a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). The binding affinity of 2’,3’-cGAMP to STING is stronger than other cyclic di-nucleotides such as c-di-GMP, 3′2′-cGAMP, 3′3′-cGAMP, and 2′2′-cGAMP with a reported K_d value of 3.79 nM compared to 1.21 μM, 1.61 μM, 1.04 μM, and 287 nM, respectively (5).

1. Zhang, X. et al. (2013) Mol Cell 51, 226-35.
2. Sun, L. et al. (2013) Science 339, 786-91.
3. Wu, J. et al. (2013) Science 339, 826-30.
4. Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
5. Zhong, B. et al. (2008) Immunity 29, 538-50.