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29255
Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X)
Activators & Inhibitors
Chemical Modulators

Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255

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Flow cytometric analysis of live human peripheral blood mononuclear cells, untreated (blue) or treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) (5 hr; green), using CD69 (FN50) Mouse mAb (PerCP-Cy5.5® Conjugate) #28633 (solid lines) or a concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PerCP-Cy5.5® Conjugate) #24589 (dashed lines).

Product Usage Information

1. Prepare the cell suspension in the appropriate cell culture medium.

2. To obtain a 1X final concentration, add the Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) directly to the cell suspension at 2 µL/mL.

3. Incubate the cells at 37°C in an atmosphere that contains CO2.

4. Harvest the cells for the immunoassay of interest.

Note: The concentration of cells and incubation time will vary depending on the desired results. This product is an ethanol solution and susceptible to evaporation. Keep tightly sealed when not in use.

Storage

Store at -20ºC tightly sealed to prevent evaporation and protected from light. This product is stable for at least 6 months when stored at -20ºC.

Product Description

Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) is an ethanol solution that contains the phorbol ester, PMA (phorbol-12-myristate-13-acetate) and the calcium ionophore, Ionomycin. When added in vitro to cell suspensions, the PMA and Ionomycin activate the cells, leading to increased cytokine production for detection by downstream immunoassays.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cy and CyDye are registered trademarks of GE Healthcare.

To Purchase # 29255S
製品番号 サイズ 価格 在庫
29255S
100 µl

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