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7722
Blue Loading Buffer Pack
バッファー & 蛍光色素
Buffer Kit

Blue Loading Buffer Pack #7722

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To Purchase # 7722S
製品番号 サイズ 価格 在庫
7722S
1 Pack

Product Includes Quantity
3X Blue Loading Buffer 8 ml
30X Reducing Agent (1.25 M DTT) 1 ml

製品概要

1. Prepare fresh 3X Reducing SDS Loading Buffer by adding 1/10 volume 30X DTT Reducing Agent to 1 volume of 3X SDS Loading Buffer.

2. Dilute 3X SDS Loading Buffer to a 1X solution using ddH2O. This product supplies enough 3X material to make 24ml of 1X solution.     

3. 培養ディッシュから培地を吸引除去し、細胞を1X PBSで洗った後、再度吸引除去してください。

4. Lyse cells by adding 1X SDS Loading Buffer (100 µl per well of 6-well plate or 500 µl per plate of 10 cm2 plate). 直ちにプレートから細胞を掻き取り、抽出物を遠心分離チューブに移してください。氷上に保持してください。

5. Sonicate for 10-15 seconds to shear DNA and reduce sample viscosity. 

6. Heat a 20 µl sample to 95-100ºC for 5 minutes; cool on ice.

7. Microcentrifuge for 2-5 minutes.

8. SDS-PAGEゲル (10 cm × 10 cm) に20 µLをアプライし、電気泳動してください。

溶液および試薬

Quantity 8 ml 3X Blue Loading Buffer and 1 ml 30X Reducing Agent

3X Blue Loading Buffer Pack: 187.5 mM Tris-HCl (pH 6.8 at 25ºC), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) bromophenol blue. (Store at room temperature.)  

30X Reducing Agent: 1.25 M dithiothreitol (DTT) (Store at -20ºC.)

保存

Store at Room Temperature and -20°C.3X Blue Loading Buffer: 187.5 mM Tris-HCl (pH 6.8 at 25°C), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) bromophenol blue. (Store at room temperature.)30X Reducing Agent: 1.25 M dithiothreitol (DTT) (Store at –20°C.)

Product Description

The Blue Loading Buffer Pack is used to lyse cells for Western blotting analysis

研究用のみ。診断手順では使用しません。

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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