Render Target: STATIC
Render Timestamp:
6/11/2026, 8:23:39 PM EDT
6/12/2026, 12:23:39 AM UTC
Commit: a854f3e2d877b833e27badd637de63a8a4794b83
XML generation date: 2026-06-05 17:29:04.981
Product last modified at: 2025-11-14T08:05:50.154Z
Cell Signaling Technologyロゴ - Extra Large
1% for the planet logo
PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® Phospho-EGF Receptor (Tyr1068) (E746-A750del Specific) Sandwich ELISA Kit #7702

Filter:
  • ELISA+

Inquiry Info. # 7702

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The PathScan® Phospho-EGF Receptor (Tyr1068) (E746-A750del Specific) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-EGF receptor (Tyr1068) (E746-A750del) mutant protein. An EGF Receptor (E746-A750del Specific) Rabbit Antibody has been coated on the microwells. After incubation with cell lysates, EGF receptor (E746-A750del) mutant protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-EGF Receptor (Tyr1068) Mouse Detection Antibody is added to detect captured phosphorylated EGF receptor (E746-A750del) mutant protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-EGF receptor (Tyr1068) (E746-A750del) mutant protein.
    Antibodies in kit are custom formulations specific to kit.

    プロトコール

    Specificity / Sensitivity

    PathScan® Phospho-EGF Receptor (Tyr1068) (E746-A750del Specific) Sandwich ELISA Kit #7702 detects endogenous levels of phospho-EGF receptor (Tyr1068) (E746-A750del) mutant protein in human cells, as shown in Figure 1. This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarcinomas that respond to EGFR inhibitors, such as gefitinib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

    Alternate Names

    avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EGF receptor; EGFR; Epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.