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PathScan® RP Total CD79B Sandwich ELISA Kit #25553

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Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

    Product Specifications

    REACTIVITY H
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP Total CD79B ELISA Kit Sandwich ELISA Kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CD79B protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with CD79B protein in a single step. The plate is then extensively washed, and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of CD79B protein. Learn more about all of your ELISA kit options here.

    *Antibodies in this kit are custom formulations specific to the kit.

    プロトコール

    Specificity / Sensitivity

    The PathScan® RP Total CD79B Sandwich ELISA Kit detects endogenous levels of CD79B, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human

    Background

    The B-cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12).

    Alternate Names

    AGM6; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; B29; CD79B; CD79B antigen; CD79b antigen (immunoglobulin-associated beta); CD79b molecule; CD79b molecule, immunoglobulin-associated beta; Ig-beta; IGB; Immunoglobulin-associated B29 protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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