Render Target: STATIC
Render Timestamp:
6/8/2026, 8:36:32 PM EDT
6/9/2026, 12:36:32 AM UTC
Commit: 33ae406c5df2bbc7a7d3c17605228fbeff8ea416
XML generation date: 2026-06-05 17:19:30.821
Product last modified at: 2026-01-29T08:02:30.428Z
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PDP - Template Name: Blocking Peptide
PDP - Template ID: *******6db2f4c

Phospho-NF-kappaB p65 (Ser276) Blocking Peptide #1437

Inquiry Info. # 1437

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    Product Information

    Product Usage Information

    For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 µl total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

    Storage

    Supplied in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol. Store at –20°C.

    Product Description

    This peptide is used to block Phospho-NF-κB p65 (Ser276) Antibody #3037 reactivity in immunohistochemistry and Western blot protocols.

    Quality Control

    The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Phospho-NF-κB p65 (Ser276) Antibody #3037 by immunohistochemistry and Western blotting.

    Background

    Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

    Alternate Names

    nfκb p65

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